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Insertion/deletion and microsatellite alteration profiles in induced pluripotent stem cells
https://repo.qst.go.jp/records/84323
https://repo.qst.go.jp/records/84323b652ca7c-ee10-4757-9f2d-74b247a1d6d2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-27 | |||||
| タイトル | ||||||
| タイトル | Insertion/deletion and microsatellite alteration profiles in induced pluripotent stem cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kamimura, Satoshi
× Kamimura, Satoshi× Suga, Tomo× Hoki, Yuko× Sunayama, Misato× Imadome, Kaori× Fujita, Mayumi× Nakamura, Miki× Araki, Ryoko× Abe, Masumi× Satoshi, Kamimura× Tomo, Suga× Yuko, Fujimori× Misato, Sunayama× Kaori, Imadome× Mayumi, Fujita× Miki, Nakamura× Ryoko, Araki× Masumi, Abe |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We here demonstrate that microsatellite (MS) alterations are elevated in both mouse and human induced pluripotent stem cells (iPSCs), but importantly we have now identified a type of human iPSC in which these alterations are considerably reduced. We aimed in our present analyses to profile the InDels in iPSC/ntESC genomes, especially in MS regions. To detect somatic de novo mutations in particular, we generated 13 independent reprogramed stem cell lines (11 iPSC and 2 ntESC lines) from an identical parent somatic cell fraction of a C57BL/6 mouse. By using this cell set with an identical genetic background, we could comprehensively detect clone-specific alterations and, importantly, experimentally validate them. The effectiveness of employing sister clones for detecting somatic de novo mutations was thereby demonstrated. We then successfully applied this approach to human iPSCs. Our results require further careful genomic analysis but make an important inroad into solving the issue of genome abnormalities in iPSCs. | |||||
| 書誌情報 |
Stem cell reports 巻 16, 号 10, p. 2503-2519, 発行日 2021-09 |
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| 出版者 | ||||||
| 出版者 | Cell Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2213-6711 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34559999 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.stemcr.2021.08.017 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S2213671121004355?via%3Dihub | |||||
