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Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants
https://repo.qst.go.jp/records/79303
https://repo.qst.go.jp/records/79303095a831d-2090-4066-b689-a4daaef03d45
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-11 | |||||
| タイトル | ||||||
| タイトル | Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kato, Yuichi
× Kato, Yuichi× Ogino, Chiaki× Hasunuma, Tomohisa× Sato, Katsuya× Ono, Yutaka× Kondo, Akihiko× Sato, Katsuya× Ono, Yutaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Microalgae are promising biofuel producers due to their high ability to produce oil photosynthetically. The oleaginous green alga Chlamydomonas sp. JSC4 is a hopeful candidate that shows high growth potential together with high oil content through activation of the starch-to-lipid biosynthesis switching mechanism. However, oil accumulation of JSC4 is significantly decreased under light/dark conditions, which is inevitable in large-scale outdoor cultivation. To overcome this critical obstacle, this study aimed to obtain a mutant strain with high oil content under light/dark conditions. Selective breeding of oil-rich Chlamydomonas mutant was performed by combining heavy ion beam mutagenesis and fluorescence-activated cell sorting (FACS). JSC4 cells seeded on agar plates were exposed to 50 Gy of the carbon ion beams (12C5+, 220 MeV) accelerated by an AVF cyclotron at TIARA, QST. Mutant cells were cultured under light/dark conditions, followed by FACS-based screening for oil-rich cells using BODIPY fluorescence and chlorophyll fluorescence as the indicators of oil and cell size, respectively. Consequently, an oil-rich mutant strain KOR1 was obtained. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2018 巻 QST-M-23, p. 82, 発行日 2020-03 |
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