{"created":"2023-05-15T14:58:27.195193+00:00","id":79303,"links":{},"metadata":{"_buckets":{"deposit":"a3734f90-f3fc-41dc-88c1-fc0ecdcaeda1"},"_deposit":{"created_by":1,"id":"79303","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"79303"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00079303","sets":["7"]},"author_link":["850129","850130","850133","850134","850135","850128","850131","850132"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"82","bibliographicVolumeNumber":"QST-M-23","bibliographic_titles":[{"bibliographic_title":"QST Takasaki Annual Report 2018"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Microalgae are promising biofuel producers due to their high ability to produce oil photosynthetically. The oleaginous green alga Chlamydomonas sp. JSC4 is a hopeful candidate that shows high growth potential together with high oil content through activation of the starch-to-lipid biosynthesis switching mechanism. However, oil accumulation of JSC4 is significantly decreased under light/dark conditions, which is inevitable in large-scale outdoor cultivation. To overcome this critical obstacle, this study aimed to obtain a mutant strain with high oil content under light/dark conditions. Selective breeding of oil-rich Chlamydomonas mutant was performed by combining heavy ion beam mutagenesis and fluorescence-activated cell sorting (FACS). JSC4 cells seeded on agar plates were exposed to 50 Gy of the carbon ion beams (12C5+, 220 MeV) accelerated by an AVF cyclotron at TIARA, QST. Mutant cells were cultured under light/dark conditions, followed by FACS-based screening for oil-rich cells using BODIPY fluorescence and chlorophyll fluorescence as the indicators of oil and cell size, respectively. Consequently, an oil-rich mutant strain KOR1 was obtained.","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Yuichi"}],"nameIdentifiers":[{"nameIdentifier":"850128","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogino, Chiaki"}],"nameIdentifiers":[{"nameIdentifier":"850129","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hasunuma, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"850130","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Katsuya"}],"nameIdentifiers":[{"nameIdentifier":"850131","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"850132","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kondo, Akihiko"}],"nameIdentifiers":[{"nameIdentifier":"850133","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Katsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"850134","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"850135","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-11"},"publish_date":"2020-03-11","publish_status":"0","recid":"79303","relation_version_is_last":true,"title":["Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:30:33.897856+00:00"}