@article{oai:repo.qst.go.jp:00079303,
 author = {Kato, Yuichi and Ogino, Chiaki and Hasunuma, Tomohisa and Sato, Katsuya and Ono, Yutaka and Kondo, Akihiko and Sato, Katsuya and Ono, Yutaka},
 journal = {QST Takasaki Annual Report 2018},
 month = {Mar},
 note = {Microalgae are promising biofuel producers due to their high ability to produce oil photosynthetically. The oleaginous green alga Chlamydomonas sp. JSC4 is a hopeful candidate that shows high growth potential together with high oil content through activation of the starch-to-lipid biosynthesis switching mechanism. However, oil accumulation of JSC4 is significantly decreased under light/dark conditions, which is inevitable in large-scale outdoor cultivation. To overcome this critical obstacle, this study aimed to obtain a mutant strain with high oil content under light/dark conditions. Selective breeding of oil-rich Chlamydomonas mutant was performed by combining heavy ion beam mutagenesis and fluorescence-activated cell sorting (FACS). JSC4 cells seeded on agar plates were exposed to 50 Gy of the carbon ion beams (12C5+, 220 MeV) accelerated by an AVF cyclotron at TIARA, QST. Mutant cells were cultured under light/dark conditions, followed by FACS-based screening for oil-rich cells using BODIPY fluorescence and chlorophyll fluorescence as the indicators of oil and cell size, respectively. Consequently, an oil-rich mutant strain KOR1 was obtained.},
 title = {Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants},
 volume = {QST-M-23},
 year = {2020}
}