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アイテム
Effects of Extremely Small Doses, 100 mGy a Day, Five Times a Week, Total of Twelve Weeks of Low-Dose Fractionated X-irradiation on Mice Hair Root
https://repo.qst.go.jp/records/77616
https://repo.qst.go.jp/records/776160265c29a-338a-4fe4-96e9-8e561574167a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-09-02 | |||||
| タイトル | ||||||
| タイトル | Effects of Extremely Small Doses, 100 mGy a Day, Five Times a Week, Total of Twelve Weeks of Low-Dose Fractionated X-irradiation on Mice Hair Root | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
J. Majima, Hideyuki
× J. Majima, Hideyuki× Ito, Hiromu× Fukushige, Tomoko× P. Indou, Hiroko× Kawabata, Yoshihiro× Ken-ichiro, Matsumoto× Nakanishi, Ikuo× Tanimoto, Akihide× Kanekura, Takuro× L. Hawkins, Clare× J. Davies, Michael× St. Clair, William× K St. Clair, Daret× Hideyuki, Majima× Hiromu, Ito× Hiroko, Indou× Kenichiro, Matsumoto× Ikuo, Nakanishi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We examined the effects of extremely small doses,100 mGy/day, 5 days/week, total of 12 weeks X-ray irradiation on the outer root sheath (ORS) cells of C3H/He mice (X-ray group). We also applied 1mM TEMPOL in drinking water in the same fractionation regimen (TEMPOL X-ray). And, these results were compared with control group (C) and TEMPOL control group. After the fractionated X-ray irradiation, the skin was removed and the cells in ORS were examined by H-E staining and immunohistochemistry methods. The cells with nuclear membrane abnormality and the cells with nucleus condensation change were examined. The X-ray irradiated mouse showed increased number of cells with nuclear membrane abnormality and those with nucleus condensation change in ORS. The pathological section was stained with 8-OHdG, 4-HNE, TUNEL, VEGF, HO-1, nitrotyrosine and PGP9.5 immunohistochemically. The results of immunohistochemistry showed that cells stained with 8-OHdG, 4-HNE, TUNEL, VEGF showed significantly increase in X Group. Electron microscope examination was also performed. The results showed that X-ray fractionation Group showed that the irregular shrunk image of ORS cells in X-ray group. Those results were not obtained in TEMPOL drinking group, suggesting TEMPOL scavenged reactive oxygen species initiated by X-ray fractionated irradiation. These results suggest that 100 mGy 60 times fractionation resulted in oxidative damage in ORS cells, and TEMPOL inhibited those effects. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 26th Annual Conference of the Society for Redox Biology and Medicine (SfRBM 2019) | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-21 | |||||
| 日付タイプ | Issued | |||||
