@misc{oai:repo.qst.go.jp:00077616,
 author = {J. Majima, Hideyuki and Ito, Hiromu and Fukushige, Tomoko and P. Indou, Hiroko and Kawabata, Yoshihiro and Ken-ichiro, Matsumoto and Nakanishi, Ikuo and Tanimoto, Akihide and Kanekura, Takuro and L. Hawkins, Clare and J. Davies, Michael and St. Clair, William and K St. Clair, Daret and Hideyuki, Majima and Hiromu, Ito and Hiroko, Indou and Kenichiro, Matsumoto and Ikuo, Nakanishi},
 month = {Nov},
 note = {We examined the effects of extremely small doses,100 mGy/day, 5 days/week, total of 12 weeks X-ray irradiation on the outer root sheath (ORS) cells of C3H/He mice (X-ray group). We also applied 1mM TEMPOL in drinking water in the same fractionation regimen (TEMPOL X-ray). And, these results were compared with control group (C) and TEMPOL control group. After the fractionated X-ray irradiation, the skin was removed and the cells in ORS were examined by H-E staining and immunohistochemistry methods. The cells with nuclear membrane abnormality and the cells with nucleus condensation change were examined. The X-ray irradiated mouse showed increased number of cells with nuclear membrane abnormality and those with nucleus condensation change in ORS. The pathological section was stained with 8-OHdG, 4-HNE, TUNEL, VEGF, HO-1, nitrotyrosine and PGP9.5 immunohistochemically. The results of immunohistochemistry showed that cells stained with 8-OHdG, 4-HNE, TUNEL, VEGF showed significantly increase in X Group. Electron microscope examination was also performed. The results showed that X-ray fractionation Group showed that the irregular shrunk image of ORS cells in X-ray group. Those results were not obtained in TEMPOL drinking group, suggesting TEMPOL scavenged reactive oxygen species initiated by X-ray fractionated irradiation. These results suggest that 100 mGy 60 times fractionation resulted in oxidative damage in ORS cells, and TEMPOL inhibited those effects., The 26th Annual Conference of the Society for Redox Biology and Medicine (SfRBM 2019)},
 title = {Effects of Extremely Small Doses, 100 mGy a Day, Five Times a Week, Total of Twelve Weeks of Low-Dose Fractionated X-irradiation on Mice Hair Root},
 year = {2019}
}