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Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells
https://repo.qst.go.jp/records/77497
https://repo.qst.go.jp/records/774979955587b-fb8e-46b3-92da-9a2171360fbc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-19 | |||||
| タイトル | ||||||
| タイトル | Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Fujimori, Akira× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Fujimori, Akira |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have treated more than 1300 patients with bone and soft tissue tumors including osteosarcoma (OS), and has achieved relatively good outcome. However, the 5-year survival rate is still less than 40%, therefore we need to develop a new combination therapy for further improving treatment results. In this study, we investigate the cell-killing mechanisms by combination of carbon-ion beam and drugs. OS cell lines, KHOS and U2OS were used. Analyses of cell cycle, apoptosis, ERK/AKT signal pathway were performed after carbon-ion beam alone or in combination with osteoporosis treating drug zoledronic acid (ZOL) or anticancer drug cisplatin (CDDP). we found that carbon-ion beam combined with ZOL significantly suppressed OS cell proliferation by arresting cell cycle, inducing apoptosis compared to X-ray or carbon-ion beam alone. In addition, the combination of carbon-ion beam and ZOL inhibited migration as well as invasion of OS cells, induced phosphorylation of MEK, and significantly inhibited ERK/AKT signal pathway related to cell proliferation, as compared with carbon-ion beam, X-ray irradiation or ZOL alone treatment. On the other hand, carbon-ion beam dose-dependently increased induction of apoptosis, and it was further increased when combined with CDDP. In addition, combined treatment with carbon-ion beam and CDDP significantly suppressed Bcl2 gene expression and further suppressed Becli1 or LC3 gene expression as compared to carbon-ion beam, X-ray irradiation alone, or X-ray irradiation and CDDP combination. Taken together, carbon-ion beam in combination with ZOL or CDDP significantly disrupts OS cells compared to carbon-ion beam alone. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第62回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-15 | |||||
| 日付タイプ | Issued | |||||
