@misc{oai:repo.qst.go.jp:00077497,
 author = {Sai, Sei and Ho Kim, Eun and Suzuki, Masao and Fujimori, Akira and Sai, Sei and Ho Kim, Eun and Suzuki, Masao and Fujimori, Akira},
 month = {Nov},
 note = {We have treated more than 1300 patients with bone and soft tissue tumors including osteosarcoma (OS), and has achieved relatively good outcome. However, the 5-year survival rate is still less than 40%, therefore we need to develop a new combination therapy for further improving treatment results. In this study, we investigate the cell-killing mechanisms by combination of carbon-ion beam and drugs.  OS cell lines, KHOS and U2OS were used. Analyses of cell cycle, apoptosis, ERK/AKT signal pathway were performed after carbon-ion beam alone or in combination with osteoporosis treating drug zoledronic acid (ZOL) or anticancer drug cisplatin (CDDP). we found that carbon-ion beam combined with ZOL significantly suppressed OS cell proliferation by arresting cell cycle, inducing apoptosis compared to X-ray or carbon-ion beam alone. In addition, the combination of carbon-ion beam and ZOL inhibited migration as well as invasion of OS cells, induced phosphorylation of MEK, and significantly inhibited ERK/AKT signal pathway related to cell proliferation, as compared with carbon-ion beam, X-ray irradiation or ZOL alone treatment. On the other hand, carbon-ion beam dose-dependently increased induction of apoptosis, and it was further increased when combined with CDDP. In addition, combined treatment with carbon-ion beam  and CDDP significantly suppressed Bcl2 gene expression and further suppressed Becli1 or LC3 gene expression as compared to carbon-ion beam, X-ray irradiation alone, or X-ray irradiation and CDDP combination. Taken together, carbon-ion beam in combination with ZOL or CDDP significantly disrupts OS cells compared to carbon-ion beam alone., 日本放射線影響学会第62回大会},
 title = {Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells},
 year = {2019}
}