{"created":"2023-05-15T14:57:06.647448+00:00","id":77497,"links":{},"metadata":{"_buckets":{"deposit":"9c34c7ac-29c7-4988-ad69-1c29af5cb95f"},"_deposit":{"created_by":1,"id":"77497","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77497"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077497","sets":["10:28"]},"author_link":["805074","805076","805075","805073","805070","805069","805071","805072"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-11-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have treated more than 1300 patients with bone and soft tissue tumors including osteosarcoma (OS), and has achieved relatively good outcome. However, the 5-year survival rate is still less than 40%, therefore we need to develop a new combination therapy for further improving treatment results. In this study, we investigate the cell-killing mechanisms by combination of carbon-ion beam and drugs.  OS cell lines, KHOS and U2OS were used. Analyses of cell cycle, apoptosis, ERK/AKT signal pathway were performed after carbon-ion beam alone or in combination with osteoporosis treating drug zoledronic acid (ZOL) or anticancer drug cisplatin (CDDP). we found that carbon-ion beam combined with ZOL significantly suppressed OS cell proliferation by arresting cell cycle, inducing apoptosis compared to X-ray or carbon-ion beam alone. In addition, the combination of carbon-ion beam and ZOL inhibited migration as well as invasion of OS cells, induced phosphorylation of MEK, and significantly inhibited ERK/AKT signal pathway related to cell proliferation, as compared with carbon-ion beam, X-ray irradiation or ZOL alone treatment. On the other hand, carbon-ion beam dose-dependently increased induction of apoptosis, and it was further increased when combined with CDDP. In addition, combined treatment with carbon-ion beam  and CDDP significantly suppressed Bcl2 gene expression and further suppressed Becli1 or LC3 gene expression as compared to carbon-ion beam, X-ray irradiation alone, or X-ray irradiation and CDDP combination. Taken together, carbon-ion beam in combination with ZOL or CDDP significantly disrupts OS cells compared to carbon-ion beam alone.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第62回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"805069","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun"}],"nameIdentifiers":[{"nameIdentifier":"805070","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"805071","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"805072","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"805073","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"805074","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"805075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"805076","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-19"},"publish_date":"2019-11-19","publish_status":"0","recid":"77497","relation_version_is_last":true,"title":["Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:40:52.816363+00:00"}