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Use of hyperpolarized magnetic resonance spectroscopy (HP-MRS) in cell and tissue engineering
https://repo.qst.go.jp/records/72922
https://repo.qst.go.jp/records/72922c6ed5387-edde-4e6c-ba37-0186fec468ef
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-09-10 | |||||
| タイトル | ||||||
| タイトル | Use of hyperpolarized magnetic resonance spectroscopy (HP-MRS) in cell and tissue engineering | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takakusagi, Yoichi
× Takakusagi, Yoichi× Takakusagi, Kaori× Inoue, Kaori× Ichikawa, Kazuhiro× 高草木 洋一× 高草木 香織 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hyperpolarized [1-13C]pyruvate magnetic resonance spectroscopy (MRS) is an emerging technique that allows noninvasive detection of metabolic conversion of [1-13C]pyruvate to [1-13C]lactate in tissues or cells depending on the over 10,000-fold enhanced 13C NMR signals. This enables chemical-shift imaging (CSI) of lactate formation from pyruvate that is significantly higher in tumors compared with normal tissues. While, recent development of tissue engineering has realized the induction of tissue-like features even in vitro, such as 3D spheroidal culture of tumor cells. This engineered tumor tissue mimetics, put in an NMR tube, may allow noninvasive assessment of the glycolytic potential in tumors grown on living animals. In this work, a strategy for hyperpolarized [1-13C]pyruvate MRS of tumor spheroids was established using murine squamous cell carcinoma (SCCVII) and PSA-negative prostate tumor cells (DU145, PC-3). Using this method, metabolic properties in cell spheroids were compared with those in normal cells cultured as monolayer. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | TERMIS - 5th Tissue Engineering and Regenerative Medicine International Society World Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2018-09-07 | |||||
| 日付タイプ | Issued | |||||
