@misc{oai:repo.qst.go.jp:00072922,
 author = {Takakusagi, Yoichi and Takakusagi, Kaori and Inoue, Kaori and Ichikawa, Kazuhiro and 高草木 洋一 and 高草木 香織},
 month = {Sep},
 note = {Hyperpolarized [1-13C]pyruvate magnetic resonance spectroscopy (MRS) is an emerging technique that allows noninvasive detection of metabolic conversion  of  [1-13C]pyruvate  to  [1-13C]lactate in tissues or cells depending on the over  10,000-fold  enhanced 13C NMR signals. This enables chemical-shift imaging (CSI) of lactate formation from pyruvate that is significantly higher in tumors compared with normal tissues. While, recent development of tissue engineering has realized the induction of tissue-like features even in vitro, such as 3D spheroidal culture of tumor cells. This engineered tumor tissue mimetics, put in an NMR tube, may allow noninvasive assessment of the glycolytic potential in tumors grown on living animals. In this work, a strategy for hyperpolarized [1-13C]pyruvate MRS of tumor spheroids was established using murine squamous cell carcinoma (SCCVII) and PSA-negative prostate tumor cells (DU145, PC-3). Using this method, metabolic properties in cell spheroids were compared with those in normal cells cultured as monolayer., TERMIS - 5th Tissue Engineering and Regenerative Medicine International Society World Congress},
 title = {Use of hyperpolarized magnetic resonance spectroscopy (HP-MRS) in cell and tissue engineering},
 year = {2018}
}