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Effects from chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes
https://repo.qst.go.jp/records/71834
https://repo.qst.go.jp/records/7183479397549-b429-4e03-95a0-6978271347e1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-10-02 | |||||
| タイトル | ||||||
| タイトル | Effects from chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
勝部, 孝則
× 勝部, 孝則× Bing, Wang× Tanaka, Kaoru× Ninomiya, Yasuharu× Vares, Guillaume× 劉強× 森田明典× Nakajima, Tetsuo× Nenoi, Mitsuru× 勝部 孝則× 王 冰× 田中 薫× 二宮 康晴× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 中島 徹夫× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Exposure to either ionizing radiation (IR) or psychological stress (PS) could cause detrimental effects on humans. A recent study showed that chronic restraint induced PS (CRIPS) could attenuate Trp53 functions and increase radiocarcinogenesis in Trp53-heterozygous mice, having a big impact on the academic and a sensational effect on the public, especially the residents living in the radioactively contaminated areas. To elucidate possible modification effects from CRIPS on radiation-induced health consequences in Trp53 wild type (Trp53wt) animals, a series of investigation with multidisciplinary analyses was done. We demonstrate here the changes in frequency of chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53wt C57BL/6J mice were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) at a dose of 4 Gy was given on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with the fluorescence in situ hybridization (FISH) probes for chromosomes 1 (green), 2 (red) and 3 (yellow). Results showed that CRIPS alone did not induce CAs, while TBI caused significant increase of CAs, mostly stable types (translocations and insertions). Intriguingly, the stable-type CAs appeared at a little high frequency in mice exposed to TBI plus CRIPS than those in mice exposed to TBI alone. Whereas, the frequency of unstable-type CAs (dicentrics and fragments) was not differ between these experimental groups (TBI+CRIPS vs. TBI). CRIPS does not seem to have a significant impact on initiation of radiocarcinogenesis of splenocytes because clastogenic effect of TBI was not enhanced by CRIPS. Further investigations elucidating possible modification effects from CRIPS on promotion and/or progression of radiocarcinogenesis of Trp53wt mice must be needed. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2015 RRS (Radiation Research Society) | |||||
| 発表年月日 | ||||||
| 日付 | 2015-09-22 | |||||
| 日付タイプ | Issued | |||||
