@misc{oai:repo.qst.go.jp:00071834,
 author = {勝部, 孝則 and Bing, Wang and Tanaka, Kaoru and Ninomiya, Yasuharu and Vares, Guillaume and 劉強 and 森田明典 and Nakajima, Tetsuo and Nenoi, Mitsuru and 勝部 孝則 and 王 冰 and 田中 薫 and 二宮 康晴 and Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ and 中島 徹夫 and 根井 充},
 month = {Sep},
 note = {Exposure to either ionizing radiation (IR) or psychological stress (PS) could cause detrimental effects on humans. A recent study showed that chronic restraint induced PS (CRIPS) could attenuate Trp53 functions and increase radiocarcinogenesis in Trp53-heterozygous mice, having a big impact on the academic and a sensational effect on the public, especially the residents living in the radioactively contaminated areas. To elucidate possible modification effects from CRIPS on radiation-induced health consequences in Trp53 wild type (Trp53wt) animals, a series of investigation with multidisciplinary analyses was done. We demonstrate here the changes in frequency of chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53wt C57BL/6J mice were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) at a dose of 4 Gy was given on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with the fluorescence in situ hybridization (FISH) probes for chromosomes 1 (green), 2 (red) and 3 (yellow). Results showed that CRIPS alone did not induce CAs, while TBI
caused significant increase of CAs, mostly stable types (translocations and insertions). Intriguingly, the stable-type CAs appeared at a little high frequency in mice exposed to TBI plus CRIPS than those in mice exposed to TBI alone. Whereas, the frequency of unstable-type CAs (dicentrics and fragments) was not differ between these experimental groups (TBI+CRIPS vs. TBI). CRIPS does not seem to have a significant impact on initiation of radiocarcinogenesis of splenocytes because clastogenic effect of TBI was not enhanced by CRIPS. Further investigations elucidating possible modification effects from CRIPS on promotion and/or progression of radiocarcinogenesis of Trp53wt mice must be needed., 2015 RRS (Radiation Research Society)},
 title = {Effects from chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes},
 year = {2015}
}