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Radioprotective and Radiomitigable Activity Screening of Natural Antioxidants and the Quantitative Evaluation
https://repo.qst.go.jp/records/71434
https://repo.qst.go.jp/records/71434a60e2520-e63f-416b-b062-571b017b3a0f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-31 | |||||
| タイトル | ||||||
| タイトル | Radioprotective and Radiomitigable Activity Screening of Natural Antioxidants and the Quantitative Evaluation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
関根, 絵美子
× 関根, 絵美子× Nakanishi, Ikuo× Ueno, Megumi× Shimokawa, Takashi× Matsumoto, Ken-ichiro× Anzai, Kazunori× Murakami, Takeshi× et.al× 関根 絵美子× 中西 郁夫× 上野 恵美× 下川 卓志× 松本 謙一郎× 安西 和紀× 村上 健 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Recently, we have invented a high-throughput screening method for the examination of radioprotective activity of chemical compounds using thymocytes (Sekine, E.; et al. Anal Chem 85:7650-53; 2013). X-irradiation of the rat thymocytes induced apoptosis, leading to a significant cell shrinkage, which could be easily detected. Methods: The rats thymocytes were treated with/without antioxidants before/after 2 Gy X-irradiation. The size of thymocytes were measured by flow cytometer 4 h after irradiation. We performed the radioprotector screening for (+)-catechin, epicatechin gallate, curcumin, resveratrol, vitamin C, vitamin E, caffeic acid, quercetin, Trolox, daidzein, genistein, carotene, and so on. Each antioxidant was applied for 1, 10, 100 μM, or 1 mM. Results and Conclusion: For the samples pretreated with antioxidants before irradiation, (+)-catechin (1 mM), epicatechin gallate (100 μ, 1 mM), curcumin (10 μM), resveratrol (100 μM), caffeic acid (100 μM, 1 mM), quercetin (1 mM), and Trolox (1 mM) efficiently protected the thymocytes against radiation-induced apoptosis. We also examined the samples treated with antioxidants after irradiation. The correlation between the radioprotective activity, as well as radiomitigable activity, and radical-scavenging rates of these antioxidants will be discussed based on the data obtained in this study. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 17th Biennial Meeting of Society for Free Radical Research International (SFRRI 2014) | |||||
| 発表年月日 | ||||||
| 日付 | 2014-03-25 | |||||
| 日付タイプ | Issued | |||||
