@misc{oai:repo.qst.go.jp:00071434,
 author = {関根, 絵美子 and Nakanishi, Ikuo and Ueno, Megumi and Shimokawa, Takashi and Matsumoto, Ken-ichiro and Anzai, Kazunori and Murakami, Takeshi and et.al and 関根 絵美子 and 中西 郁夫 and 上野 恵美 and 下川 卓志 and 松本 謙一郎 and 安西 和紀 and 村上 健},
 month = {Mar},
 note = {Background: Recently, we have invented a high-throughput screening method for the examination of radioprotective activity of chemical compounds using thymocytes (Sekine, E.; et al. Anal Chem 85:7650-53; 2013). X-irradiation of the rat thymocytes induced apoptosis, leading to a significant cell shrinkage, which could be easily detected. 
Methods: The rats thymocytes were treated with/without antioxidants before/after 2 Gy X-irradiation.  The size of thymocytes were measured by flow cytometer 4 h after irradiation. We performed the radioprotector screening for (+)-catechin, epicatechin gallate, curcumin, resveratrol, vitamin C, vitamin E, caffeic acid, quercetin, Trolox, daidzein, genistein, carotene, and so on. Each antioxidant was applied for 1, 10, 100 μM, or 1 mM. 
Results and Conclusion: For the samples pretreated with antioxidants before irradiation, (+)-catechin (1 mM), epicatechin gallate (100 μ, 1 mM), curcumin (10 μM), resveratrol (100 μM), caffeic acid (100 μM, 1 mM), quercetin (1 mM), and Trolox (1 mM) efficiently protected the thymocytes against radiation-induced apoptosis. We also examined the samples treated with antioxidants after irradiation. The correlation between the radioprotective activity, as well as radiomitigable activity, and radical-scavenging rates of these antioxidants will be discussed based on the data obtained in this study., 17th Biennial Meeting of Society for Free Radical Research International (SFRRI 2014)},
 title = {Radioprotective and Radiomitigable Activity Screening of Natural Antioxidants and the Quantitative Evaluation},
 year = {2014}
}