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Regulation of IL-9R expression in T-cell lymphoma cell line
https://repo.qst.go.jp/records/71346
https://repo.qst.go.jp/records/7134622b44c1e-a015-4634-98ae-972b74bea609
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-11-14 | |||||
| タイトル | ||||||
| タイトル | Regulation of IL-9R expression in T-cell lymphoma cell line | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shang, Yi
× Shang, Yi× Kakinuma, Shizuko× Nishimura, Mayumi× Kobayashi, Yoshiro× Shimada, Yoshiya× et.al× 臺野 和広× 今岡 達彦× 飯塚 大輔× 西村 まゆみ× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | ABSTRACT Background: Interleukin-9 (IL-9) is a multifunctional cytokine secreted from Th2 cells. Besides its role in the immune and inflammatory responses, its growth promoting and antiapoptotic activities in multiple transformed cells suggest a potential role in tumorigenesis. The receptor of IL-9 (IL-9R) is not usually expressed in normal thymocytes except for fetal or newborn thymocytes. We previously reported that IL-9R is unusually overexpressed and activated in radiation-induced mouse T-cell lymphomas (TL) which is an animal model of human T-cell acute lymphoblastic leukemia. These results suggest that IL-9R could be considered as an oncofetal antigen which could be used as a biomarker for diagnosis or treatment of T-cell leukemias. We have extended our study on the transcriptional regulation mechanism of IL-9R gene (Il9r). \nMaterials and Methods: We established an Il9r-highly-expressing TL cell line and examined the promoter activity with luciferase reporter assay. \nResults and Discussion: We found that promoter regulatory region was located at a 5' AT-rich region from -410 bp to -487 bp upstream translation start site. The results from electrophoretic mobility shift assay (EMSA), liquid chromatography mass spectrometric mass spectrometry (LC-MS/MS) and chromatin immunoprecipitation (ChIP) assay revealed that the histone H4 is hyperacetylated and nucleolin was associated to the promoter region in lymphoma cells. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | KIDS workshop 2009 in NIRS - IAEA NIRS Joint Workshop & NIRS Symposium on Radiation Protection for Children | |||||
| 発表年月日 | ||||||
| 日付 | 2009-12-16 | |||||
| 日付タイプ | Issued | |||||
