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Comparison on the repair of potentially lethal damage between exposure to low- and high-LET radiations from Kinetics and fidelity of chromosome rejoining in normal human fibroblasts
https://repo.qst.go.jp/records/70973
https://repo.qst.go.jp/records/70973f04a9a2d-e844-47ab-8fd4-da2d8d3ff54b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-11-09 | |||||
| タイトル | ||||||
| タイトル | Comparison on the repair of potentially lethal damage between exposure to low- and high-LET radiations from Kinetics and fidelity of chromosome rejoining in normal human fibroblasts | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hua, Liu Cui
× Hua, Liu Cui× Kawata, Tetsuya× Shigematsu, Naoyuki× George, Kerry× A, Cucinotta Francesca× 劉 翠華× 川田 哲也× ジョージ ケリー× クシノッタ フランク |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Potentially lethal damage (PLD) and its repair (PLDR) were studied in confluent human fibroblasts by analyzing the kinetics of chromosome break rejoining in X-ray or heavy- ion irradiated cells that were either held in non-cycling G0 phase of the cell cycle or forced to proliferate immediately after irradiation. Fusion premature chromosome condensation (PCC) was combined with fluorescence in situ hybridization (FISH) to study chromosomal aberrations in interphase. The rejoining kinetics of PCC breaks was similar for each culture condition after both X-ray and heavy-ion irradiation. However, after 6 Gy X-rays, the misrepair in cycling cells was 1.36, 2.8 times higher than that in non-cycling cells. Twelve hours after exposure to 2 Gy of 55keV/um silicon ions the misrepair in cycling cells was 0.56, only 2 times higher than that in non-cycling cells. Whereas, after exposure to 2 Gy 200 keV/um or 440 keV/um iron ion beams misrepair was similar in non-cycling and cycling cells. . Since the majority of repair in G0/G1 occurs via the non-homologous end joining process (NHEJ), increased PLDR in X-ray and silicon-ion irradiated cells may result from improved cell cycle specific rejoining fidelity through the NHEJ pathway, which is not the case in high-LET iron-ion irradiated cells. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Radiation Research Society 58th Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2012-10-05 | |||||
| 日付タイプ | Issued | |||||
