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Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma
https://repo.qst.go.jp/records/70860
https://repo.qst.go.jp/records/70860433516a1-42ff-4743-b1ea-682f986e63c4
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-09-03 | |||||
| タイトル | ||||||
| タイトル | Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ueno, Megumi
× Ueno, Megumi× Nyuui, Minako× Nakanishi, Ikuo× Anzai, Kazunori× Ozawa, Toshihiko× Matsumoto, Kenichiro× Uto, Yoshihiro× 上野 恵美× 乳井 美奈子× 中西 郁夫× 安西 和紀× 小澤 俊彦× 松本 謙一郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It was reported that reactive oxygen species (ROS) were generated by heating an aqueous solution containing oxygen. The GSH dependent reduction of a nitroxyl radical, TEMPOL, is accelerated temperature dependently. This reaction can be restricted by adding SOD or by deoxygenating the reaction mixture, therefore used as an index of O2- generation. In this presentation, the hyperthermia-induced ROS scavenging ability of rat plasma was tested. A reaction mixture containing 0.1 mM nitroxyl radical (TEMPOL) and 1 mM GSH was prepared using 100 mM phosphate buffer (PB) containing 0.05 mM DTPA. The reaction mixture was kept in a screw-top vial, and was incubated in a water bath at 37 or 44[deg]C. The time course of the EPR signal of TEMPOL in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). The similar experiment was done using rat plasma instead of 100 mM PB. Effect of BSA and/or ascorbic acid was tested. ROS generation in an aqueous fluid containing oxygen at hyperthermal temperature was demonstrated. Adding 0.5% albumin to the reaction mixture could almost cancel the ROS generation at 37[deg]C; however, 0.5% albumin could not cancel the ROS at 44[deg]C, i.e. hyperthermal temperature. Ascorbic acid above 0.02 mM also could almost cancel ROS at 37[deg]C, however, 0.03 mM could cancel ROS at 44[deg]C. Hyperthermia induced ROS in rat plasma can be mostly canceled by relatively high concentration of albumin and ascorbic acid in the plasma. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ICHO & JCTM 2012 (The 11th International Congress of Hyperthermic Oncology and The 29th Japanese Congress of Thermal Medicine) | |||||
| 発表年月日 | ||||||
| 日付 | 2012-08-31 | |||||
| 日付タイプ | Issued | |||||
