@misc{oai:repo.qst.go.jp:00070860,
 author = {Ueno, Megumi and Nyuui, Minako and Nakanishi, Ikuo and Anzai, Kazunori and Ozawa, Toshihiko and Matsumoto, Kenichiro and Uto, Yoshihiro and 上野 恵美 and 乳井 美奈子 and 中西 郁夫 and 安西 和紀 and 小澤 俊彦 and 松本 謙一郎},
 month = {Aug},
 note = {It was reported that reactive oxygen species (ROS) were generated by heating an aqueous solution containing oxygen. The GSH dependent reduction of a nitroxyl radical, TEMPOL, is accelerated temperature dependently. This reaction can be restricted by adding SOD or by deoxygenating the reaction mixture, therefore used as an index of O2- generation. In this presentation, the hyperthermia-induced ROS scavenging ability of rat plasma was tested.
A reaction mixture containing 0.1 mM nitroxyl radical (TEMPOL) and 1 mM GSH was prepared using 100 mM phosphate buffer (PB) containing 0.05 mM DTPA. The reaction mixture was kept in a screw-top vial, and was incubated in a water bath at 37 or 44[deg]C. The time course of the EPR signal of TEMPOL in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). The similar experiment was done using rat plasma instead of 100 mM PB. Effect of BSA and/or ascorbic acid was tested.
ROS generation in an aqueous fluid containing oxygen at hyperthermal temperature was demonstrated. Adding 0.5% albumin to the reaction mixture could almost cancel the ROS generation at 37[deg]C; however, 0.5% albumin could not cancel the ROS at 44[deg]C, i.e. hyperthermal temperature. Ascorbic acid above 0.02 mM also could almost cancel ROS at 37[deg]C, however, 0.03 mM could cancel ROS at 44[deg]C.
Hyperthermia induced ROS in rat plasma can be mostly canceled by relatively high concentration of albumin and ascorbic acid in the plasma., ICHO & JCTM 2012 (The 11th International Congress of Hyperthermic Oncology and The 29th Japanese Congress of Thermal Medicine)},
 title = {Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma},
 year = {2012}
}