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The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1
https://repo.qst.go.jp/records/70741
https://repo.qst.go.jp/records/7074101c7889c-abd3-4b46-93c6-086f4e0808df
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-15 | |||||
| タイトル | ||||||
| タイトル | The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Mayumi
× Fujita, Mayumi× Imadome, Kaori× Shoji, Yoshimi× Imai, Takashi× 藤田 真由美× 今留 香織× 荘司 好美× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | [Objectives] The aim of this study was to compare the effects of X-ray or carbon-ion (C-ion) irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1. [Methods] MIAPaCa-2 and PANC-1 was irradiated with either X-ray (0, 2, or 4 Gy) or C-ion (0, 0.5, 1, 2, and 4 Gy). We examined the migration, invasion, protease expression and invasiveness in response to protease inhibitors and/or ROCK inhibitors of irradiated cells, and the activation of Rac1 and Rho. [Results] X-ray irradiation enhanced MIAPaCa-2 and PANC-1 invasion via MMP-2, whereas, Carbon-ion irradiation reduced MIAPaCa-2 invasion but increased PANC-1 invasion via activation of serine proteases (SerP). Treatment of MMP inhibitor or SerP inhibitor failed to decrease the radiation-enhanced MIAPaCa-2 or PANC-1 invasion accompanied by mesenchymal–amoeboid transition. ROCK inhibitor plus those protease inhibitor suppressed the enhanced invasiveness, suggested that both modes of motility were significant in MIAPACa-2 and PANC-1. We further found that irradiation affects the activity of small GTPase, Rac1 and RhoA, the key factors involved in two modes of motility. Rac1 was activated in X-ray-irradiated MIAPaCa-2, whereas, both Rac1 and RhoA activities were diminished in C-ion-irradiated MIAPaCa-2. In contrast, RhoA was activated in C-ion-irradiated PANC-1. [Conclusions] X-ray and C-ion irradiation show differential effects on the invasive potential of MIAPaCa-2 and PANC-1 cells with corresponding alterations in the MMP-2 activity or SerP activity, and also the activation of Rac1 and Rho signalings. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 2nd Japan-China Symposium on Cancer Research | |||||
| 発表年月日 | ||||||
| 日付 | 2012-05-11 | |||||
| 日付タイプ | Issued | |||||
