{"created":"2023-05-15T14:51:44.971609+00:00","id":70741,"links":{},"metadata":{"_buckets":{"deposit":"4df1c5eb-29a1-4275-95f0-d2c86a4b5b2e"},"_deposit":{"created_by":1,"id":"70741","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70741"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070741","sets":["10:28"]},"author_link":["694915","694917","694912","694911","694916","694914","694913","694910"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-05-11","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Objectives] The aim of this study was to compare the effects of X-ray or carbon-ion (C-ion) irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1.\n[Methods] MIAPaCa-2 and PANC-1 was irradiated with either X-ray (0, 2, or 4 Gy) or C-ion (0, 0.5, 1, 2, and 4 Gy). We examined the migration, invasion, protease expression and invasiveness in response to protease inhibitors and/or ROCK inhibitors of irradiated cells, and the activation of Rac1 and Rho.\n[Results] X-ray irradiation enhanced MIAPaCa-2 and PANC-1 invasion via MMP-2, whereas, Carbon-ion irradiation reduced MIAPaCa-2 invasion but increased PANC-1 invasion via activation of serine proteases (SerP). Treatment of MMP inhibitor or SerP inhibitor failed to decrease the radiation-enhanced MIAPaCa-2 or PANC-1 invasion accompanied by mesenchymal–amoeboid transition. ROCK inhibitor plus those protease inhibitor suppressed the enhanced invasiveness, suggested that both modes of motility were significant in MIAPACa-2 and PANC-1. We further found that irradiation affects the activity of small GTPase, Rac1 and RhoA, the key factors involved in two modes of motility. Rac1 was activated in X-ray-irradiated MIAPaCa-2, whereas, both Rac1 and RhoA activities were diminished in C-ion-irradiated MIAPaCa-2. In contrast, RhoA was activated in C-ion-irradiated PANC-1.\n[Conclusions] X-ray and C-ion irradiation show differential effects on the invasive potential of MIAPaCa-2 and PANC-1 cells with corresponding alterations in the MMP-2 activity or SerP activity, and also the activation of Rac1 and Rho signalings.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 2nd Japan-China Symposium on Cancer Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"694910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imadome, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"694911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shoji, Yoshimi"}],"nameIdentifiers":[{"nameIdentifier":"694912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"694913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤田 真由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今留 香織","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荘司 好美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694917","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-15"},"publish_date":"2012-05-15","publish_status":"0","recid":"70741","relation_version_is_last":true,"title":["The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:59:23.270949+00:00"}