@misc{oai:repo.qst.go.jp:00070741,
 author = {Fujita, Mayumi and Imadome, Kaori and Shoji, Yoshimi and Imai, Takashi and 藤田 真由美 and 今留 香織 and 荘司 好美 and 今井 高志},
 month = {May},
 note = {[Objectives] The aim of this study was to compare the effects of X-ray or carbon-ion (C-ion) irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1.
[Methods] MIAPaCa-2 and PANC-1 was irradiated with either X-ray (0, 2, or 4 Gy) or C-ion (0, 0.5, 1, 2, and 4 Gy). We examined the migration, invasion, protease expression and invasiveness in response to protease inhibitors and/or ROCK inhibitors of irradiated cells, and the activation of Rac1 and Rho.
[Results] X-ray irradiation enhanced MIAPaCa-2 and PANC-1 invasion via MMP-2, whereas, Carbon-ion irradiation reduced MIAPaCa-2 invasion but increased PANC-1 invasion via activation of serine proteases (SerP). Treatment of MMP inhibitor or SerP inhibitor failed to decrease the radiation-enhanced MIAPaCa-2 or PANC-1 invasion accompanied by mesenchymal–amoeboid transition. ROCK inhibitor plus those protease inhibitor suppressed the enhanced invasiveness, suggested that both modes of motility were significant in MIAPACa-2 and PANC-1. We further found that irradiation affects the activity of small GTPase, Rac1 and RhoA, the key factors involved in two modes of motility. Rac1 was activated in X-ray-irradiated MIAPaCa-2, whereas, both Rac1 and RhoA activities were diminished in C-ion-irradiated MIAPaCa-2. In contrast, RhoA was activated in C-ion-irradiated PANC-1.
[Conclusions] X-ray and C-ion irradiation show differential effects on the invasive potential of MIAPaCa-2 and PANC-1 cells with corresponding alterations in the MMP-2 activity or SerP activity, and also the activation of Rac1 and Rho signalings., The 2nd Japan-China Symposium on Cancer Research},
 title = {The effects of irradiation on invasive potential of human pancreatic cancer cell lines, MIAPaCa-2 and PANC-1},
 year = {2012}
}