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Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging
https://repo.qst.go.jp/records/70259
https://repo.qst.go.jp/records/70259f7330cb3-44a9-434d-bd61-7b5e4c41a664
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-28 | |||||
| タイトル | ||||||
| タイトル | Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ｕ, Ｗｉｎｎ Ａｕｎｇ
× Ｕ, Ｗｉｎｎ Ａｕｎｇ× 長谷川, 純崇× 古川, 高子× 佐賀, 恒夫× Ｕ Ｗｉｎｎ Ａｕｎｇ× 長谷川 純崇× 古川 高子× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of study was to analyze regulable p53 transgene expression in real time and treatment response in a timely manner, using non-invasive imaging techniques. We constructed a doxycycline-regulated bidirectional vector harboring a reporter gene encoding red fluorescence protein (RFP) and a tumor-suppressor-protein (wild-type p53), and demonstrated their inducible and coordinated expression, p53 mediated-downstream signaling, and its effects both in vitro and in vivo. Next, we evaluated glucose utilization in cells with and without exogenous p53 expression by measuring the cellular uptakes of [14C]FDG, indirectly verified the transferred p53 overexpression in xenograft tumor via simultaneous expression of RFP detectable with an in vivo optical imaging system, and subsequently performed FDG-PET imaging. Cells or tumors with p53 overexpression exhibited decreased uptake of [14C]FDG in cellular assay and [18F]FDG in PET imaging. Thus, by coupling with bidirectional vector, controllable p53 transfer was achieved and the capability of FDG-PET to assess the therapeutic response to p53 gene therapy was evidently confirmed, which may have an impact on the improvement of p53 gene therapy. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第69回日本癌学会学術総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-24 | |||||
| 日付タイプ | Issued | |||||
