@misc{oai:repo.qst.go.jp:00070259,
 author = {Ｕ, Ｗｉｎｎ Ａｕｎｇ and 長谷川, 純崇 and 古川, 高子 and 佐賀, 恒夫 and Ｕ Ｗｉｎｎ Ａｕｎｇ and 長谷川 純崇 and 古川 高子 and 佐賀 恒夫},
 month = {Sep},
 note = {The aim of study was to analyze regulable p53 transgene expression in real time and treatment response in a timely manner, using non-invasive imaging techniques. We constructed a doxycycline-regulated bidirectional vector harboring a reporter gene encoding red fluorescence protein (RFP) and a tumor-suppressor-protein (wild-type p53), and demonstrated their inducible and coordinated expression, p53 mediated-downstream signaling, and its effects both in vitro and in vivo. Next, we evaluated glucose utilization in cells with and without exogenous p53 expression by measuring the cellular uptakes of [14C]FDG, indirectly verified the transferred p53 overexpression in xenograft tumor via simultaneous expression of RFP detectable with an in vivo optical imaging system, and subsequently performed FDG-PET imaging. Cells or tumors with p53 overexpression exhibited decreased uptake of [14C]FDG in cellular assay and [18F]FDG in PET imaging. Thus, by coupling with bidirectional vector, controllable p53 transfer was achieved and the capability of FDG-PET to assess the therapeutic response to p53 gene therapy was evidently confirmed, which may have an impact on the improvement of p53 gene therapy., 第69回日本癌学会学術総会},
 title = {Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging},
 year = {2010}
}