{"created":"2023-05-15T14:51:23.666324+00:00","id":70259,"links":{},"metadata":{"_buckets":{"deposit":"9aa383cc-7d24-44e9-a37d-74e4d92ec23b"},"_deposit":{"created_by":1,"id":"70259","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70259"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070259","sets":["10:28"]},"author_link":["689870","689874","689873","689867","689871","689869","689872","689868"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-09-24","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The aim of study was to analyze regulable p53 transgene expression in real time and treatment response in a timely manner, using non-invasive imaging techniques. We constructed a doxycycline-regulated bidirectional vector harboring a reporter gene encoding red fluorescence protein (RFP) and a tumor-suppressor-protein (wild-type p53), and demonstrated their inducible and coordinated expression, p53 mediated-downstream signaling, and its effects both in vitro and in vivo. Next, we evaluated glucose utilization in cells with and without exogenous p53 expression by measuring the cellular uptakes of [14C]FDG, indirectly verified the transferred p53 overexpression in xenograft tumor via simultaneous expression of RFP detectable with an in vivo optical imaging system, and subsequently performed FDG-PET imaging. Cells or tumors with p53 overexpression exhibited decreased uptake of [14C]FDG in cellular assay and [18F]FDG in PET imaging. Thus, by coupling with bidirectional vector, controllable p53 transfer was achieved and the capability of FDG-PET to assess the therapeutic response to p53 gene therapy was evidently confirmed, which may have an impact on the improvement of p53 gene therapy.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第69回日本癌学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ｕ, Ｗｉｎｎ Ａｕｎｇ"}],"nameIdentifiers":[{"nameIdentifier":"689867","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川, 純崇"}],"nameIdentifiers":[{"nameIdentifier":"689868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川, 高子"}],"nameIdentifiers":[{"nameIdentifier":"689869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀, 恒夫"}],"nameIdentifiers":[{"nameIdentifier":"689870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｕ Ｗｉｎｎ Ａｕｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川 純崇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689874","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-09-28"},"publish_date":"2010-09-28","publish_status":"0","recid":"70259","relation_version_is_last":true,"title":["Evaluating regulable transferred-p53 expression and therapeutic response in tumor model by optical and FDG-PET imaging"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:05:02.186986+00:00"}