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Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody
https://repo.qst.go.jp/records/70255
https://repo.qst.go.jp/records/7025569415399-aa0a-4f5e-9acf-a43eb784963b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-27 | |||||
| タイトル | ||||||
| タイトル | Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sogawa, Chizuru
× Sogawa, Chizuru× Tsuji, Atsushi× Furukawa, Takako× Koizumi, Mitsuru× Kurosawa, Yoshikazu× Saga, Tsuneo× et.al× 曽川 千鶴× 辻 厚至× 古川 高子× 小泉 満× 黒澤 良和× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: Over-expression or mutation of EGFR is identified in several cancers including hepatocellular carcinoma (HCC). We are trying to develop a novel imaging method to detect EGFR over-expression at an early stage of cancers including HCC. In this study, we evaluated the radiolabeled human anti-EGFR monoclonal antibody for this purpose. \nMaterial and Methods: High EGFR-expressing HCC cell lines were selected from four cell lines (Hep-G2, HuH-7, SK-Hep1 and Li-7) by western blot analysis and immunohistochemistry. Cancer cells (2x106) were inoculated subcutaneously into BALB/c-nu/nu mice with Matrigel. Anti-EGFR monoclonal antibody (048-006, IgG1 subclass) were labeled with 125I using the chloramine-T or 111In using CHX-A"-DTPA as a bifunctional chelate. The in vitro character of the radiolabeled 048-006 was evaluated by cell binding, competitive inhibition and internalization assays, and then in vivo character was assessed by biodistribution and planar imaging in tumor model mice. \nResults: Western blotting analysis revealed that HuH-7 showed the highest EGFR expression among four cell lines tested. EGFR was expressed on the cell membrane of HuH-7 by immunohistochemistry. After subcutaneous inoculation with Matrigel, HuH-7 cells successfully formed tumor xenografts showing membranous EGFR expression. In vitro studies showed that 125I-048-006 and 111In-048-006 specifically bound to HuH-7 with the affinity constants of 9.3x108M-1 and 8.0x108M-1, respectively. This antibody was internalized after binding to EGFR. 111In-048-006 highly accumulated in xenografted tumors (highest tumor uptake of 21.24+/-5.84 %ID/gram obtained at 48 hours after injection), and the tumor xenograft was clearly visualized by planar imaging. \nConclusion: This radiolabeled human anti-EGFR monoclonal antibody 048-006 would be promising for the imaging HCC. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Federation of Nuclear Medicine and Biology 2010 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-23 | |||||
| 日付タイプ | Issued | |||||
