@misc{oai:repo.qst.go.jp:00070255,
 author = {Sogawa, Chizuru and Tsuji, Atsushi and Furukawa, Takako and Koizumi, Mitsuru and Kurosawa, Yoshikazu and Saga, Tsuneo and et.al and 曽川 千鶴 and 辻 厚至 and 古川 高子 and 小泉 満 and 黒澤 良和 and 佐賀 恒夫},
 month = {Sep},
 note = {Introduction: Over-expression or mutation of EGFR is identified in several cancers including hepatocellular carcinoma (HCC). We are trying to develop a novel imaging method to detect EGFR over-expression at an early stage of cancers including HCC. In this study, we evaluated the radiolabeled human anti-EGFR monoclonal antibody for this purpose.
\nMaterial and Methods: High EGFR-expressing HCC cell lines were selected from four cell lines (Hep-G2, HuH-7, SK-Hep1 and Li-7) by western blot analysis and immunohistochemistry. Cancer cells (2x106) were inoculated subcutaneously into BALB/c-nu/nu mice with Matrigel. Anti-EGFR monoclonal antibody (048-006, IgG1 subclass) were labeled with 125I using the chloramine-T or 111In using CHX-A"-DTPA as a bifunctional chelate. The in vitro character of the radiolabeled 048-006 was evaluated by cell binding, competitive inhibition and internalization assays, and then in vivo character was assessed by biodistribution and planar imaging in tumor model mice. 
\nResults: Western blotting analysis revealed that HuH-7 showed the highest EGFR expression among four cell lines tested. EGFR was expressed on the cell membrane of HuH-7 by immunohistochemistry. After subcutaneous inoculation with Matrigel, HuH-7 cells successfully formed tumor xenografts showing membranous EGFR expression. In vitro studies showed that 125I-048-006 and 111In-048-006 specifically bound to HuH-7 with the affinity constants of 9.3x108M-1 and 8.0x108M-1, respectively. This antibody was internalized after binding to EGFR. 111In-048-006 highly accumulated in xenografted tumors (highest tumor uptake of 21.24+/-5.84 %ID/gram obtained at 48 hours after injection), and the tumor xenograft was clearly visualized by planar imaging. 
\nConclusion: This radiolabeled human anti-EGFR monoclonal antibody 048-006 would be promising for the imaging HCC., World Federation of Nuclear Medicine and Biology 2010},
 title = {Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody},
 year = {2010}
}