{"created":"2023-05-15T14:51:23.482052+00:00","id":70255,"links":{},"metadata":{"_buckets":{"deposit":"3ea26def-3c36-487b-915b-31e448484ead"},"_deposit":{"created_by":1,"id":"70255","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70255"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070255","sets":["10:28"]},"author_link":["689839","689846","689840","689837","689847","689838","689843","689848","689836","689844","689845","689842","689841"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-09-23","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: Over-expression or mutation of EGFR is identified in several cancers including hepatocellular carcinoma (HCC). We are trying to develop a novel imaging method to detect EGFR over-expression at an early stage of cancers including HCC. In this study, we evaluated the radiolabeled human anti-EGFR monoclonal antibody for this purpose.\n\\nMaterial and Methods: High EGFR-expressing HCC cell lines were selected from four cell lines (Hep-G2, HuH-7, SK-Hep1 and Li-7) by western blot analysis and immunohistochemistry. Cancer cells (2x106) were inoculated subcutaneously into BALB/c-nu/nu mice with Matrigel. Anti-EGFR monoclonal antibody (048-006, IgG1 subclass) were labeled with 125I using the chloramine-T or 111In using CHX-A\"-DTPA as a bifunctional chelate. The in vitro character of the radiolabeled 048-006 was evaluated by cell binding, competitive inhibition and internalization assays, and then in vivo character was assessed by biodistribution and planar imaging in tumor model mice. \n\\nResults: Western blotting analysis revealed that HuH-7 showed the highest EGFR expression among four cell lines tested. EGFR was expressed on the cell membrane of HuH-7 by immunohistochemistry. After subcutaneous inoculation with Matrigel, HuH-7 cells successfully formed tumor xenografts showing membranous EGFR expression. In vitro studies showed that 125I-048-006 and 111In-048-006 specifically bound to HuH-7 with the affinity constants of 9.3x108M-1 and 8.0x108M-1, respectively. This antibody was internalized after binding to EGFR. 111In-048-006 highly accumulated in xenografted tumors (highest tumor uptake of 21.24+/-5.84 %ID/gram obtained at 48 hours after injection), and the tumor xenograft was clearly visualized by planar imaging. \n\\nConclusion: This radiolabeled human anti-EGFR monoclonal antibody 048-006 would be promising for the imaging HCC.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"World Federation of Nuclear Medicine and Biology 2010","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sogawa, Chizuru"}],"nameIdentifiers":[{"nameIdentifier":"689836","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"689837","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"689838","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koizumi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"689839","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kurosawa, Yoshikazu"}],"nameIdentifiers":[{"nameIdentifier":"689840","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"689841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"689842","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽川 千鶴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小泉 満","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"黒澤 良和","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689847","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689848","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-09-27"},"publish_date":"2010-09-27","publish_status":"0","recid":"70255","relation_version_is_last":true,"title":["Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:05:04.983855+00:00"}