WEKO3
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ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy
https://repo.qst.go.jp/records/70086
https://repo.qst.go.jp/records/700865399f4f5-e7d8-46fd-9da7-a2f0282dec8d
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-04-26 | |||||
タイトル | ||||||
タイトル | ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Fujimori, Akira
× Fujimori, Akira× Kato, Takamitsu× Okayasu, Ryuichi× 藤森 亮× 加藤 宝光× 岡安 隆一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Comprehensive search for human cellular response to ionizing radiation (IR) is a helpful strategy to understand the molecular basis under tumor radiotherapy. In a previous study using HiCEP, we found that ASPM (Abnormal spindle-like microcephaly-associated) or the most common-type microcephaly (MCPH5) gene is selectively down-regulated by IR. Here we first demonstrate that down-regulation of ASPM by siRNA synergistically enhanced radiosensitivity in some immortalized human cell lines. Both constant-field gel electrophoreses and gamma-H2AX foci assays exhibited impaired DNA double-strand breaks (DSB) in the cells treated with the ASPM-specific siRNA. Greater extent of chromosomal abnormality was also found in the siRNA-treated cells than in negative controls. In addition, this IR-sensitization by ASPM knock-down was abrogated in a DNA-PK deficient glioblastoma cells. The results indicacated that ASPM has a role in the NHEJ pathway of DNA double-strand break repair. In clinic, ASPM would be a promising target molecule for cancer therapies as well as a prognotic biomarker for the cancer treatment. |
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会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | AACR annual meeting 2010 | |||||
発表年月日 | ||||||
日付 | 2010-04-21 | |||||
日付タイプ | Issued |