@misc{oai:repo.qst.go.jp:00070086,
 author = {Fujimori, Akira and Kato, Takamitsu and Okayasu, Ryuichi and 藤森 亮 and 加藤 宝光 and 岡安 隆一},
 month = {Apr},
 note = {Comprehensive search for human cellular response to ionizing radiation (IR) is a helpful strategy to understand the molecular basis under tumor radiotherapy. In a previous study using HiCEP, we found that ASPM (Abnormal spindle-like microcephaly-associated) or the most common-type microcephaly (MCPH5) gene is selectively down-regulated by IR. Here we first demonstrate that down-regulation of ASPM by siRNA synergistically enhanced radiosensitivity in some immortalized human cell lines. Both constant-field gel electrophoreses and gamma-H2AX foci assays exhibited impaired DNA double-strand breaks (DSB) in the cells treated with the ASPM-specific siRNA. Greater extent of chromosomal abnormality was also found in the siRNA-treated cells than in negative controls. In addition, this IR-sensitization by ASPM knock-down was abrogated in a DNA-PK deficient glioblastoma cells. The results indicacated that ASPM has a role in the NHEJ pathway of DNA double-strand break repair.
In clinic, ASPM would be a promising target molecule for cancer therapies as well as a prognotic biomarker for the cancer treatment., AACR annual meeting 2010},
 title = {ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy},
 year = {2010}
}