{"created":"2023-05-15T14:51:16.102834+00:00","id":70086,"links":{},"metadata":{"_buckets":{"deposit":"68c29739-8009-44d9-a61e-ea662dd1f223"},"_deposit":{"created_by":1,"id":"70086","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70086"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070086","sets":["10:28"]},"author_link":["688243","688248","688245","688246","688244","688247"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-04-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Comprehensive search for human cellular response to ionizing radiation (IR) is a helpful strategy to understand the molecular basis under tumor radiotherapy. In a previous study using HiCEP, we found that ASPM (Abnormal spindle-like microcephaly-associated) or the most common-type microcephaly (MCPH5) gene is selectively down-regulated by IR. Here we first demonstrate that down-regulation of ASPM by siRNA synergistically enhanced radiosensitivity in some immortalized human cell lines. Both constant-field gel electrophoreses and gamma-H2AX foci assays exhibited impaired DNA double-strand breaks (DSB) in the cells treated with the ASPM-specific siRNA. Greater extent of chromosomal abnormality was also found in the siRNA-treated cells than in negative controls. In addition, this IR-sensitization by ASPM knock-down was abrogated in a DNA-PK deficient glioblastoma cells. The results indicacated that ASPM has a role in the NHEJ pathway of DNA double-strand break repair.\nIn clinic, ASPM would be a promising target molecule for cancer therapies as well as a prognotic biomarker for the cancer treatment.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"AACR annual meeting 2010","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"688243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Takamitsu"}],"nameIdentifiers":[{"nameIdentifier":"688244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"688245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤 宝光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688248","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-04-26"},"publish_date":"2010-04-26","publish_status":"0","recid":"70086","relation_version_is_last":true,"title":["ASPM is a novel participant in DNA double-strand break repair and a potential target for radiotherapy"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:06:59.475215+00:00"}