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Relationship between telomere length and radiosensitivity of human cancer cell lines induced by heavy ion irradiation.
https://repo.qst.go.jp/records/70084
https://repo.qst.go.jp/records/70084dd1126ad-7c8b-424e-a506-dc1984b2cbd5
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-04-21 | |||||
| タイトル | ||||||
| タイトル | Relationship between telomere length and radiosensitivity of human cancer cell lines induced by heavy ion irradiation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Zhang, Hong
× Zhang, Hong× Wang, Yanling× Bing, Wang× Tanaka, Kaoru× Li, Ning× Zhou, Xin× 王 冰× 田中 薫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In this work, we investigate the possible role of BRCA1 in telomerase regulation in response to ionizing radiation (IR). The two human breast cancer cell lines expressing either BRCA-null (HCC1937) or BRCA1-wild type (MCF-7) cell lines were exposed to 4Gy of carbon ions. At different time point following radiation, cell cycle distribution was detected by flow cytometric analysis; telomerase activity was determined by PCR ELISA; the expression of hTERT mRNA, a catalytic subunit of telomerase complex, was determined by RT-PCR; the expression of telomerase protein, BRCA1 and p21 was examined by Western blotting. The results showed that a strong down-regulation of telomerase activity, telomerase expression and hTERT mRNA was observed in MCF-7 cells following irradiation. At the same time, IR induced a significant G2 arrest in these cells. In contrast, neither down-regulation of telomerase activity nor G2 block was detected in HCC1937. Furthermore, the decline in telomerase expression was rescued when G2 checkpoint was abrogated in MCF-7. The data suggest that inhibition of telomerase is largely BRCA1-dependent, which is mediated by G2 checkpoint. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 平成21年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究発表会 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-04-20 | |||||
| 日付タイプ | Issued | |||||
