@misc{oai:repo.qst.go.jp:00070084,
 author = {Zhang, Hong and Wang, Yanling and Bing, Wang and Tanaka, Kaoru and Li, Ning and Zhou, Xin and 王 冰 and 田中 薫},
 month = {Apr},
 note = {In this work, we investigate the possible role of BRCA1 in telomerase
regulation in response to ionizing radiation (IR). The two human breast
cancer cell lines expressing either BRCA-null (HCC1937) or BRCA1-wild type
(MCF-7) cell lines were exposed to 4Gy of carbon ions. At different time
point following radiation, cell cycle distribution was detected by flow
cytometric analysis; telomerase activity was determined by PCR ELISA; the
expression of hTERT mRNA, a catalytic subunit of telomerase complex, was
determined by RT-PCR; the expression of telomerase protein, BRCA1 and p21
was examined by Western blotting. The results showed that a strong
down-regulation of telomerase activity, telomerase expression and hTERT mRNA
was observed in MCF-7 cells following irradiation. At the same time, IR
induced a significant G2 arrest in these cells. In contrast, neither
down-regulation of telomerase activity nor G2 block was detected in HCC1937.
Furthermore, the decline in telomerase expression was rescued when G2
checkpoint was abrogated in MCF-7. The data suggest that inhibition of
telomerase is largely BRCA1-dependent, which is mediated by G2 checkpoint., 平成21年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究発表会},
 title = {Relationship between telomere length and radiosensitivity of human cancer cell lines induced by heavy ion irradiation.},
 year = {2010}
}