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Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors
https://repo.qst.go.jp/records/69563
https://repo.qst.go.jp/records/6956344035f26-a5b6-49ec-8178-e318185e2cb3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-31 | |||||
| タイトル | ||||||
| タイトル | Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
U, Winn Aung
× U, Winn Aung× Hasegawa, Sumitaka× Koshikawa, Michiko× Furukawa, Takako× Saga, Tsuneo× Ｕ Ｗｉｎｎ Ａｕｎｇ× 長谷川 純崇× 越川 道子× 古川 高子× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a MRI reporter, and RFP gene to visualize transgene by dual modality. In cellular T2-weighted MRI, cells transfected with FHC plasmid showed lower signal intensity compared to the control cells. Moreover, after in vivo EGT, the plasmid-injected region in the tumor showed low signal intensity on T2-weighted MRI. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第67回日本癌学会学術総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2008-10-30 | |||||
| 日付タイプ | Issued | |||||
