@misc{oai:repo.qst.go.jp:00069563,
 author = {U, Winn Aung and Hasegawa, Sumitaka and Koshikawa, Michiko and Furukawa, Takako and Saga, Tsuneo and Ｕ Ｗｉｎｎ Ａｕｎｇ and 長谷川 純崇 and 越川 道子 and 古川 高子 and 佐賀 恒夫},
 month = {Oct},
 note = {In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a MRI reporter, and RFP gene to visualize transgene by dual modality. In cellular T2-weighted MRI, cells transfected with FHC plasmid showed lower signal intensity compared to the control cells. Moreover, after in vivo EGT, the plasmid-injected region in the tumor showed low signal intensity on T2-weighted MRI. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate., 第67回日本癌学会学術総会},
 title = {Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors},
 year = {2008}
}