{"created":"2023-05-15T14:50:53.331487+00:00","id":69563,"links":{},"metadata":{"_buckets":{"deposit":"052fe00c-e9e9-4211-aae5-6d92e0fb5b41"},"_deposit":{"created_by":1,"id":"69563","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69563"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069563","sets":["10:28"]},"author_link":["682785","682791","682782","682783","682787","682790","682786","682788","682784","682789"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-10-30","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a MRI reporter, and RFP gene to visualize transgene by dual modality. In cellular T2-weighted MRI, cells transfected with FHC plasmid showed lower signal intensity compared to the control cells. Moreover, after in vivo EGT, the plasmid-injected region in the tumor showed low signal intensity on T2-weighted MRI. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第67回日本癌学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"U, Winn Aung"}],"nameIdentifiers":[{"nameIdentifier":"682782","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hasegawa, Sumitaka"}],"nameIdentifiers":[{"nameIdentifier":"682783","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koshikawa, Michiko"}],"nameIdentifiers":[{"nameIdentifier":"682784","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"682785","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"682786","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｕ Ｗｉｎｎ Ａｕｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682787","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川 純崇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682788","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"越川 道子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682789","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682791","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-31"},"publish_date":"2008-10-31","publish_status":"0","recid":"69563","relation_version_is_last":true,"title":["Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:13:02.384848+00:00"}