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Prolonged expression changes of cell-cycle related genes in murine tumor models treated with carbon ion irradiation
https://repo.qst.go.jp/records/69122
https://repo.qst.go.jp/records/69122c39af0a9-df7f-4ade-a7e6-42948e44142b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-02 | |||||
| タイトル | ||||||
| タイトル | Prolonged expression changes of cell-cycle related genes in murine tumor models treated with carbon ion irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Iwakawa, Mayumi
× Iwakawa, Mayumi× Imadome, Kaori× Nojiri, Kazunori× Tamaki, Tomoaki× Sakai, Minako× Nakawatari, Miyako× Moritake, Takashi× Yanagisawa, Mitsuru× Nakamura, Etsuko× Tsujii, Hirohiko× Imai, Takashi× 岩川 眞由美× 今留 香織× 野尻 和典× 田巻 倫明× 酒井 美奈子× 中渡 美也子× 盛武 敬× 柳澤 充× 辻井 博彦× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objective: To elucidate the in vivo biological effects induced by carbon-ion irradiation using comprehensive expression analysis. Materials and Methods: We examined gene expression changes after carbon-ion (C-ion) irradiation (290 MeV/m, SOBP 6 cm middle, 50 kev/m) with a single dose of 30 Gy in four mouse tumors (NR-S1, SCCVII, NFSa, and #8520) transplanted into the hind legs of C3H/HeNrs mice, using 44K single-color oligo-microarrays at 6 hours (h), 1 day, and 3 days after irradiation. Gamma rays of 30 Gy and 50 Gy were used as a reference beam. Identification of C-ion-responsive genes was based on a false discovery rate of < 5% using the Wilcoxon test (P < 0.001) and the Benjamini-Hochberg correction. Results: In all tumors, the level of expression of several tens of genes, including Ccl3, Ccng1, Cd80, Cdkn1a, Cxcl2, IL7r, Lrdd, Mgmt, Mmp8, and Polk, was significantly altered 6 h and day 1 following C-ion irradiation. At day 3, several hundred genes, many of which are also classified as stress-response or cell-communication genes, including Tnfrsf5, Ikbke, Ifi202b, and Icam1, were upregulated following C-ion irradiation. The expression level of the majority of these genes was similar following -ray treatment, although the change was not as extensive and intertumor variance was apparent. Several genes, including Ikbke, Serpina3n, and Saa3, responded differentially following C-ion irradiation than after -ray irradiation. Pathological investigation and immunohistochemical analysis of Cdkn1a revealed cell cycle arrest with mitotic catastrophe in tumors irradiated by C-ions. Conclusions: This study revealed significant C-ion induced up-regulation of stress-responsive and cell-communication genes common to different tumor types. These findings provide evidence for the efficacy of this modality for the treatment of local tumors. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第66回日本癌学会学術総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-10-05 | |||||
| 日付タイプ | Issued | |||||
