@misc{oai:repo.qst.go.jp:00069122,
 author = {Iwakawa, Mayumi and Imadome, Kaori and Nojiri, Kazunori and Tamaki, Tomoaki and Sakai, Minako and Nakawatari, Miyako and Moritake, Takashi and Yanagisawa, Mitsuru and Nakamura, Etsuko and Tsujii, Hirohiko and Imai, Takashi and 岩川 眞由美 and 今留 香織 and 野尻 和典 and 田巻 倫明 and 酒井 美奈子 and 中渡 美也子 and 盛武 敬 and 柳澤 充 and 辻井 博彦 and 今井 高志},
 month = {Oct},
 note = {Objective: To elucidate the in vivo biological effects induced by carbon-ion irradiation using comprehensive expression analysis.
Materials and Methods: We examined gene expression changes after carbon-ion (C-ion) irradiation (290 MeV/m, SOBP 6 cm middle, 50 kev/&#61549;m) with a single dose of 30 Gy in four mouse tumors (NR-S1, SCCVII, NFSa, and #8520) transplanted into the hind legs of C3H/HeNrs mice, using 44K single-color oligo-microarrays at 6 hours (h), 1 day, and 3 days after irradiation. Gamma rays of 30 Gy and 50 Gy were used as a reference beam. Identification of C-ion-responsive genes was based on a false discovery rate of < 5% using the Wilcoxon test (P < 0.001) and the Benjamini-Hochberg correction. 
Results: In all tumors, the level of expression of several tens of genes, including Ccl3, Ccng1, Cd80, Cdkn1a, Cxcl2, IL7r, Lrdd, Mgmt, Mmp8, and Polk, was significantly altered 6 h and day 1 following C-ion irradiation. At day 3, several hundred genes, many of which are also classified as stress-response or cell-communication genes, including Tnfrsf5, Ikbke, Ifi202b, and Icam1, were upregulated following C-ion irradiation. The expression level of the majority of these genes was similar following &#61543;-ray treatment, although the change was not as extensive and intertumor variance was apparent. Several genes, including Ikbke, Serpina3n, and Saa3, responded differentially following C-ion irradiation than after &#61543;-ray irradiation. Pathological investigation and immunohistochemical analysis of Cdkn1a revealed cell cycle arrest with mitotic catastrophe in tumors irradiated by C-ions. 
Conclusions: This study revealed significant C-ion induced up-regulation of stress-responsive and cell-communication genes common to different tumor types. These findings provide evidence for the efficacy of this modality for the treatment of local tumors., 第66回日本癌学会学術総会},
 title = {Prolonged expression changes of cell-cycle related genes in murine tumor models treated with carbon ion irradiation},
 year = {2007}
}