WEKO3
アイテム
EFNA1, a radioresistant marker, detected in a murine tumor model by gamma and carbon ion irradiation
https://repo.qst.go.jp/records/69110
https://repo.qst.go.jp/records/691104b684566-da9e-40f6-b39c-04546f3148eb
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-01 | |||||
| タイトル | ||||||
| タイトル | EFNA1, a radioresistant marker, detected in a murine tumor model by gamma and carbon ion irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nojiri, Kazunori
× Nojiri, Kazunori× Iwakawa, Mayumi× Ichikawa, Yasushi× Imadome, Kaori× Sakai, Minako× Nakawatari, Miyako× Ishikawa, Kenichi× Ishikawa, Atsuko× Togo, Shinji× Tsujii, Hirohiko× Shimada, Hiroshi× Imai, Takashi× 野尻 和典× 岩川 眞由美× 市川 靖史× 今留 香織× 酒井 美奈子× 中渡 美也子× 石川 顕一× 石川 敦子× 辻井 博彦× 嶋田 紘× 今井 高志 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Using single-color oligo-microarrays, we analyzed the gene expression profiles of two murine squamous cell carcinomas: NR-S1 (radioresistant) and SCCVII (radiosensitive), after irradiation with 137-Cs gamma rays at doses of 30, 50 and 70 Gy or 290 MeV/u carbon ions at a dose of 30 Gy. Potential genes related to radiosensitivity were selected by comparing the expression values before and after irradiation (with both gamma rays and carbon ions) using a filter for at least 1.5-fold changes. Furthermore, candidate genes which had significantly different ratio values between the two tumors (P < 0.05) were detected by unpaired Student's t-tests. Subsequent analysis by quantitative reverse-transcription polymerase chain reaction (RT-PCR) confirmed our microarray data. Protein expression and function were examined by immunohistochemical studies. Results: Four genes, Efna1, Sprr1a, Srgap3 and Xrra1, were selected as potential genes related to radioresistance after gamma and carbon ion irradiation. RT-PCR confirmed that Efna1 was induced in radioresistant NR-S1. Efna1, a proangiogenic factor, was expressed in the cytoplasm of tumor cells and significant increases in microvascular density were observed in the radioresistant NR-S1. Conclusions: We found that Efna1 may be a potential molecule related to radioresistance in murine tumors. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 14th European Cancer Conference, ESTRO26 meeting, European Society for therapeutic radiology and oncology | |||||
| 発表年月日 | ||||||
| 日付 | 2007-09-27 | |||||
| 日付タイプ | Issued | |||||
