@misc{oai:repo.qst.go.jp:00069110,
 author = {Nojiri, Kazunori and Iwakawa, Mayumi and Ichikawa, Yasushi and Imadome, Kaori and Sakai, Minako and Nakawatari, Miyako and Ishikawa, Kenichi and Ishikawa, Atsuko and Togo, Shinji and Tsujii, Hirohiko and Shimada, Hiroshi and Imai, Takashi and 野尻 和典 and 岩川 眞由美 and 市川 靖史 and 今留 香織 and 酒井 美奈子 and 中渡 美也子 and 石川 顕一 and 石川 敦子 and 辻井 博彦 and 嶋田 紘 and 今井 高志},
 month = {Sep},
 note = {Using single-color oligo-microarrays, we analyzed the gene expression profiles of two murine squamous cell carcinomas: NR-S1 (radioresistant) and SCCVII (radiosensitive), after irradiation with 137-Cs gamma rays at doses of 30, 50 and 70 Gy or 290 MeV/u carbon ions at a dose of 30 Gy. Potential genes related to radiosensitivity were selected by comparing the expression values before and after irradiation (with both gamma rays and carbon ions) using a filter for at least 1.5-fold changes. Furthermore, candidate genes which had significantly different ratio values between the two tumors (P < 0.05) were detected by unpaired Student's t-tests. Subsequent analysis by quantitative reverse-transcription polymerase chain reaction (RT-PCR) confirmed our microarray data. Protein expression and function were examined by immunohistochemical studies.
Results: Four genes, Efna1, Sprr1a, Srgap3 and Xrra1, were selected as potential genes related to radioresistance after gamma and carbon ion irradiation. RT-PCR confirmed that Efna1 was induced in radioresistant NR-S1. Efna1, a proangiogenic factor, was expressed in the cytoplasm of tumor cells and significant increases in microvascular density were observed in the radioresistant NR-S1.
Conclusions: We found that Efna1 may be a potential molecule related to radioresistance in murine tumors., The 14th European Cancer Conference, ESTRO26 meeting, European Society for therapeutic radiology and oncology},
 title = {EFNA1, a radioresistant marker, detected in a murine tumor model by gamma and carbon ion irradiation},
 year = {2007}
}