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Low dose radiation inducible vectors for cancer gene therapy
https://repo.qst.go.jp/records/68897
https://repo.qst.go.jp/records/68897213164eb-435a-4f20-8f78-303a15a80006
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-03-30 | |||||
| タイトル | ||||||
| タイトル | Low dose radiation inducible vectors for cancer gene therapy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nenoi, Mitsuru
× Nenoi, Mitsuru× Nakajima, Tetsuo× Taki, Keiko× 根井 充× 中島 徹夫× 瀧 景子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although tumor suppressor protein p53 is induced by low doses (<1 Gy) of radiation, there have been only a few reports indicating potential utilization of a p53-target gene promoter, such as that of the p21 gene. This is mainly because the transiently transfected promoter of p53-target genes is not much sensitive to radiation. The purpose of this study is to evaluate radiation sensitivity of the p53-target gene promoter integrated into host cell chromosomes by use of adeno-associated virus (rAAV) vectors. \nMethods: The response of the p21 gene promoter to low-dose radiation was tested when transduced into a human breast cancer cell line MCF-7 by use of rAAV vectors. The suicide gene HSVtk linked to the p21 gene promoter was transduced into MCF-7 cells by rAAV, and their radiation-sensitivity was examined. \nResults: It was shown that the p21 gene promoter transduced by rAAV vectors was more highly radiation-responsive than that transiently transfected by electroporation. A significant induction of the p21 gene promoter by radiation of low doses down to 0.2 Gy was observed. When cells were transduced with the p21 gene promoter-driven HSVtk gene by rAAV vector, they were siginificantly sensitized to repetitive treatment with low dose radiation (1 Gy) in the presence of the prodrug ganciclovir. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2nd International Congress of Molecular Medicine | |||||
| 発表年月日 | ||||||
| 日付 | 2007-03-26 | |||||
| 日付タイプ | Issued | |||||
