@misc{oai:repo.qst.go.jp:00068897,
 author = {Nenoi, Mitsuru and Nakajima, Tetsuo and Taki, Keiko and 根井 充 and 中島 徹夫 and 瀧 景子},
 month = {Mar},
 note = {Objectives: In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although tumor suppressor protein p53 is induced by low doses (<1 Gy) of radiation, there have been only a few reports indicating potential utilization of a p53-target gene promoter, such as that of the p21 gene. This is mainly because the transiently transfected promoter of p53-target genes is not much sensitive to radiation. The purpose of this study is to evaluate radiation sensitivity of the p53-target gene promoter integrated into host cell chromosomes by use of adeno-associated virus (rAAV) vectors.
\nMethods: The response of the p21 gene promoter to low-dose radiation was tested when transduced into a human breast cancer cell line MCF-7 by use of rAAV vectors. The suicide gene HSVtk linked to the p21 gene promoter was transduced into MCF-7 cells by rAAV, and their radiation-sensitivity was examined. 
\nResults: It was shown that the p21 gene promoter transduced by rAAV vectors was more highly radiation-responsive than that transiently transfected by electroporation. A significant induction of the p21 gene promoter by radiation of low doses down to 0.2 Gy was observed. When cells were transduced with the p21 gene promoter-driven HSVtk gene by rAAV vector, they were siginificantly sensitized to repetitive treatment with low dose radiation (1 Gy) in the presence of the prodrug ganciclovir., 2nd International Congress of Molecular Medicine},
 title = {Low dose radiation inducible vectors for cancer gene therapy},
 year = {2007}
}