{"created":"2023-05-15T14:50:22.810685+00:00","id":68897,"links":{},"metadata":{"_buckets":{"deposit":"819a18b7-1387-4ae7-992f-5c9e8b85e015"},"_deposit":{"created_by":1,"id":"68897","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68897"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068897","sets":["10:28"]},"author_link":["676199","676201","676200","676198","676196","676197"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-03-26","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although tumor suppressor protein p53 is induced by low doses (<1 Gy) of radiation, there have been only a few reports indicating potential utilization of a p53-target gene promoter, such as that of the p21 gene. This is mainly because the transiently transfected promoter of p53-target genes is not much sensitive to radiation. The purpose of this study is to evaluate radiation sensitivity of the p53-target gene promoter integrated into host cell chromosomes by use of adeno-associated virus (rAAV) vectors.\n\\nMethods: The response of the p21 gene promoter to low-dose radiation was tested when transduced into a human breast cancer cell line MCF-7 by use of rAAV vectors. The suicide gene HSVtk linked to the p21 gene promoter was transduced into MCF-7 cells by rAAV, and their radiation-sensitivity was examined. \n\\nResults: It was shown that the p21 gene promoter transduced by rAAV vectors was more highly radiation-responsive than that transiently transfected by electroporation. A significant induction of the p21 gene promoter by radiation of low doses down to 0.2 Gy was observed. When cells were transduced with the p21 gene promoter-driven HSVtk gene by rAAV vector, they were siginificantly sensitized to repetitive treatment with low dose radiation (1 Gy) in the presence of the prodrug ganciclovir.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"2nd International Congress of Molecular Medicine","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"676196","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakajima, Tetsuo"}],"nameIdentifiers":[{"nameIdentifier":"676197","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taki, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"676198","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676199","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中島 徹夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676200","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧 景子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676201","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Low dose radiation inducible vectors for cancer gene therapy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Low dose radiation inducible vectors for cancer gene therapy"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-03-30"},"publish_date":"2007-03-30","publish_status":"0","recid":"68897","relation_version_is_last":true,"title":["Low dose radiation inducible vectors for cancer gene therapy"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:20:50.939020+00:00"}