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DEVELOPMENTAL CHANGES IN THE PROLIFERATIVE AND DIFFERENTIATIVE ACTIVITIES OF EPIDERMAL MELANOCYTES FROM SLATY MOUSE IN SERUM-FREE CULTURE
https://repo.qst.go.jp/records/68168
https://repo.qst.go.jp/records/68168a97ca782-4d55-4a05-9c82-0b699c56ad54
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-09-27 | |||||
| タイトル | ||||||
| タイトル | DEVELOPMENTAL CHANGES IN THE PROLIFERATIVE AND DIFFERENTIATIVE ACTIVITIES OF EPIDERMAL MELANOCYTES FROM SLATY MOUSE IN SERUM-FREE CULTURE | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirobe, Tomohisa
× Hirobe, Tomohisa× Wakamatsu, Kazumasa× Ito, Shosuke× Kawa, Yoko× Soma, Yosinao× Mizoguchi, Masako× 廣部 知久 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Developmental changes in the proliferative and differentiative activities of neonatal epidermal melanocytes derived from wild-type mice (Slt/Slt) at the slaty (slt) locus and from congenic mice mutant (slt/slt) at that locus were investigated in serum-free primary culture with or without addition of L-tyrosine (Tyr). Incubation with added L-Tyr inhibited the proliferation of both Slt/Slt and slt/slt melanocytes in a concentration-dependent manner and that inhibition was gradually augmented as the donor mice aged. In contrast, the differentiation of melanocytes in slt/slt mice was inhibited compared to that in Slt/Slt mice. Tyrosinase-related protein (TRP)-1, TRP-2 and c-kit were expressed in the slt/slt melanocytes, though the expression of TRP-2 in the slt/slt melanocytes was reduced to 30-50% than that in Slt/Slt melanocytes. L-Tyr induced the differentiation of slt/slt melanocytes as the age of the donor mice advanced. The content of the eumelanin marker, pyrrole-2,3,5-tricarboxylic acid in slt/slt melanocytes was reduced compared to that of Slt/Slt melanocytes. However, the content of the pheomelanin marker, 4-amino-3-hydroxyphenylalanine in slt/slt melanocytes was similar to that of Slt/Slt melanocytes. L-Tyr increased the content of eumelanin and pheomelanin 2- to 3-fold in cultured cells derived from 0.5-, 3.5- and 7.5-day-old mice both in Slt/Slt and slt/slt. However, the content of eumelanin and pheomelanin in culture media derived from slt/slt mice was greatly increased after treatment with L-Tyr, especially from 7.5-day-old mice. These results suggest that the differentiation of slt/slt melanocytes is induced by L-Tyr as the age of the donor mice advanced, even though eumelanin and pheomelanin fail to accumulate and are released from them at all ages of skin development. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第１９回国際色素細胞学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2005-09-22 | |||||
| 日付タイプ | Issued | |||||
