{"created":"2023-05-15T14:49:47.514163+00:00","id":68168,"links":{},"metadata":{"_buckets":{"deposit":"0832b459-3294-4847-873e-acbab0a09f27"},"_deposit":{"created_by":1,"id":"68168","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68168"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068168","sets":["10:28"]},"author_link":["669319","669316","669321","669322","669318","669320","669317"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2005-09-22","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Developmental changes in the proliferative and differentiative activities of neonatal epidermal melanocytes derived from wild-type mice (Slt/Slt) at the slaty (slt) locus and from congenic mice mutant (slt/slt) at that locus were investigated in serum-free primary culture with or without addition of L-tyrosine (Tyr). Incubation with added L-Tyr inhibited the proliferation of both Slt/Slt and slt/slt melanocytes in a concentration-dependent manner and that inhibition was gradually augmented as the donor mice aged. In contrast, the differentiation of melanocytes in slt/slt mice was inhibited compared to that in Slt/Slt mice. Tyrosinase-related protein (TRP)-1, TRP-2 and c-kit were expressed in the slt/slt melanocytes, though the expression of TRP-2 in the slt/slt melanocytes was reduced to 30-50% than that in Slt/Slt melanocytes. L-Tyr induced the differentiation of slt/slt melanocytes as the age of the donor mice advanced. The content of the eumelanin marker, pyrrole-2,3,5-tricarboxylic acid in slt/slt melanocytes was reduced compared to that of Slt/Slt melanocytes. However, the content of the pheomelanin marker, 4-amino-3-hydroxyphenylalanine in slt/slt melanocytes was similar to that of Slt/Slt melanocytes. L-Tyr increased the content of eumelanin and pheomelanin 2- to 3-fold in cultured cells derived from 0.5-, 3.5- and 7.5-day-old mice both in Slt/Slt and slt/slt. However, the content of eumelanin and pheomelanin in culture media derived from slt/slt mice was greatly increased after treatment with L-Tyr, especially from 7.5-day-old mice. These results suggest that the differentiation of slt/slt melanocytes is induced by L-Tyr as the age of the donor mice advanced, even though eumelanin and pheomelanin fail to accumulate and are released from them at all ages of skin development.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第１９回国際色素細胞学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"669316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Kazumasa"}],"nameIdentifiers":[{"nameIdentifier":"669317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Shosuke"}],"nameIdentifiers":[{"nameIdentifier":"669318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawa, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"669319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Soma, Yosinao"}],"nameIdentifiers":[{"nameIdentifier":"669320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizoguchi, Masako"}],"nameIdentifiers":[{"nameIdentifier":"669321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"669322","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"DEVELOPMENTAL CHANGES IN THE PROLIFERATIVE AND DIFFERENTIATIVE ACTIVITIES OF EPIDERMAL MELANOCYTES FROM SLATY MOUSE IN SERUM-FREE CULTURE","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DEVELOPMENTAL CHANGES IN THE PROLIFERATIVE AND DIFFERENTIATIVE ACTIVITIES OF EPIDERMAL MELANOCYTES FROM SLATY MOUSE IN SERUM-FREE CULTURE"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-09-27"},"publish_date":"2005-09-27","publish_status":"0","recid":"68168","relation_version_is_last":true,"title":["DEVELOPMENTAL CHANGES IN THE PROLIFERATIVE AND DIFFERENTIATIVE ACTIVITIES OF EPIDERMAL MELANOCYTES FROM SLATY MOUSE IN SERUM-FREE CULTURE"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:29:18.862044+00:00"}