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マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導
https://repo.qst.go.jp/records/67866
https://repo.qst.go.jp/records/678660e5e9401-76af-4def-b70b-b4eebc70cfe6
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-09-13 | |||||
| タイトル | ||||||
| タイトル | マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
鈴木, 桂子
× 鈴木, 桂子× 田中, 泉× 榑松, 文子× 薬丸, 晴子× 伊古田, 暢夫× 石原, 弘× 鈴木 桂子× 田中 泉× 槫松 文子× 薬丸 晴子× 伊古田 暢夫× 石原 弘 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heme oxygenase-1 (HO-1) is a cytoprotective enzyme which is transcriptionally activated by various stimuli. To investigate the potential of various phytochemicals to contribute to cytoprotection, we measured transcriptional activation of HO-1 gene in RAW264.7 mouse monocyte-macrophage cells. After the cells were incubated with EGCG, resveratrol, ellagic acid, and caffeic acid phenethyl ester (CAPE), a total RNA fraction was prepared from the cells. The amount of the mRNA of HO-1 was quantified by RT-PCR method. Among the chemicals CAPE (a component of propolis) was found to be a most potent activator, and increased HO-1 mRNA as 45 times at 2 uM. When caffeic acid ethyl ester (CAEE), chlorogenic acid and rosmarinic acid were tested, all being caffeic acid esters, 2 uM of CAEE stimulated the HO-1 transcription as 20 times although chlorogenic acid and rosmarinic had no activity. Caffeic acid and phenethyl alcohol could not activate HO-1 transcription. Therefore caffeic acid phenethyl ester itself is assigned as the activator. Curcumin and ethyl ferulate were also able to increase HO-1 mRNA at 2 uM as 6.4 and 7.2 respectively. When J774.1 mouse macrophage cell line and Hep G2 human hepatocarcinoma cell line were treated with 2 uM of CAPE, J774.1 cells exhibited 33 times augmentation of HO-1 mRNA, but no activation was observed in Hep G2 cells. Therefore it was confirmed that the activation is specific to macrophage cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第77回日本生化学会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2004-10-16 | |||||
| 日付タイプ | Issued | |||||
