@misc{oai:repo.qst.go.jp:00067866,
 author = {鈴木, 桂子 and 田中, 泉 and 榑松, 文子 and 薬丸, 晴子 and 伊古田, 暢夫 and 石原, 弘 and 鈴木 桂子 and 田中 泉 and 槫松 文子 and 薬丸 晴子 and 伊古田 暢夫 and 石原 弘},
 month = {Oct},
 note = {Heme oxygenase-1 (HO-1) is a cytoprotective enzyme which is transcriptionally activated by various stimuli.  To investigate the potential of various phytochemicals to contribute to cytoprotection, we measured transcriptional activation of HO-1 gene in RAW264.7 mouse monocyte-macrophage cells.  After the cells were incubated with EGCG, resveratrol, ellagic acid, and caffeic acid phenethyl ester (CAPE), a total RNA fraction was prepared from the cells.  The amount of the mRNA of HO-1 was quantified by RT-PCR method.  Among the chemicals CAPE (a component of propolis) was found to be a most potent activator, and increased HO-1 mRNA as 45 times at 2 uM.  When caffeic acid ethyl ester (CAEE), chlorogenic acid and rosmarinic acid were tested, all being caffeic acid esters, 2 uM of CAEE stimulated the HO-1 transcription as 20 times although chlorogenic acid and rosmarinic had no activity.  Caffeic acid and phenethyl alcohol could not activate HO-1 transcription.  Therefore caffeic acid phenethyl ester itself is assigned as the activator.  Curcumin and ethyl ferulate were also able to increase HO-1 mRNA at 2 uM as 6.4 and 7.2 respectively.  When J774.1 mouse macrophage cell line and Hep G2 human hepatocarcinoma cell line were treated with 2 uM of CAPE, J774.1 cells exhibited 33 times augmentation of HO-1 mRNA, but no activation was observed in Hep G2 cells.  Therefore it was confirmed that the activation is specific to macrophage cells., 第77回日本生化学会大会},
 title = {マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導},
 year = {2004}
}