{"created":"2023-05-15T14:49:33.850379+00:00","id":67866,"links":{},"metadata":{"_buckets":{"deposit":"aee48205-4853-4e13-aca3-db1412581526"},"_deposit":{"created_by":1,"id":"67866","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67866"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067866","sets":["10:28"]},"author_link":["666684","666690","666681","666692","666683","666687","666686","666685","666691","666688","666682","666689"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2004-10-16","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heme oxygenase-1 (HO-1) is a cytoprotective enzyme which is transcriptionally activated by various stimuli.  To investigate the potential of various phytochemicals to contribute to cytoprotection, we measured transcriptional activation of HO-1 gene in RAW264.7 mouse monocyte-macrophage cells.  After the cells were incubated with EGCG, resveratrol, ellagic acid, and caffeic acid phenethyl ester (CAPE), a total RNA fraction was prepared from the cells.  The amount of the mRNA of HO-1 was quantified by RT-PCR method.  Among the chemicals CAPE (a component of propolis) was found to be a most potent activator, and increased HO-1 mRNA as 45 times at 2 uM.  When caffeic acid ethyl ester (CAEE), chlorogenic acid and rosmarinic acid were tested, all being caffeic acid esters, 2 uM of CAEE stimulated the HO-1 transcription as 20 times although chlorogenic acid and rosmarinic had no activity.  Caffeic acid and phenethyl alcohol could not activate HO-1 transcription.  Therefore caffeic acid phenethyl ester itself is assigned as the activator.  Curcumin and ethyl ferulate were also able to increase HO-1 mRNA at 2 uM as 6.4 and 7.2 respectively.  When J774.1 mouse macrophage cell line and Hep G2 human hepatocarcinoma cell line were treated with 2 uM of CAPE, J774.1 cells exhibited 33 times augmentation of HO-1 mRNA, but no activation was observed in Hep G2 cells.  Therefore it was confirmed that the activation is specific to macrophage cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第77回日本生化学会大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 桂子"}],"nameIdentifiers":[{"nameIdentifier":"666681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 泉"}],"nameIdentifiers":[{"nameIdentifier":"666682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"榑松, 文子"}],"nameIdentifiers":[{"nameIdentifier":"666683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"薬丸, 晴子"}],"nameIdentifiers":[{"nameIdentifier":"666684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊古田, 暢夫"}],"nameIdentifiers":[{"nameIdentifier":"666685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原, 弘"}],"nameIdentifiers":[{"nameIdentifier":"666686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 桂子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中 泉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"槫松 文子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"薬丸 晴子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊古田 暢夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原 弘","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666692","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-09-13"},"publish_date":"2004-09-13","publish_status":"0","recid":"67866","relation_version_is_last":true,"title":["マウス単球-マクロファージ細胞株RAW264.7における、カフェイン酸フェネチルエステルによる強力なヘムオキシゲナーゼ-1遺伝子誘導"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:32:50.578897+00:00"}