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XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強
https://repo.qst.go.jp/records/65241
https://repo.qst.go.jp/records/6524113f82cc8-f584-477c-a4eb-8fb3ca509f48
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-31 | |||||
| タイトル | ||||||
| タイトル | XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
崔, 星
× 崔, 星× 若井, 俊文× Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ× 王, 冰× 根井, 充× 上條, 岳彦× 白井, 敏之× 鎌田, 正× 崔 星× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 王 冰× 根井 充× 上條 岳彦× 白井 敏之× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Here we report the role of XRCC4 in cancer stem cell (CSC) properties and radiosensitivity of CSCs to X-ray and carbon ion beam. FACS analysis showed that the CSCs (CD133+, CD44+/ESA+) were significantly altered in XRCC4 KO cells compared to HCT116-WT cells. The number of colony and spheroid formed from CSCs were significantly higher compared to that from non-CSCs in XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. The proportion of CSCs was more extremely increased in XRCC4 KO cells compared to HCT116-WT cells after X-ray irradiation. Survival fraction analysis showed that the doses at D10 levels of CSCs sorted from XRCC4-KO and HCT116-WT cells were 1.4 and 4.2 Gy for X-ray and 1.0 and 1.9 Gy for carbon ion beam, respectively. A much more large number and large-sized gamma-H2AX foci were observed in CSCs sorted from XRCC4 KO cells compared to that from HCT116-WT cells, after 24 h carbon ion beam compared to X-ray irradiation. In conclusion, lack of XRCC4 significantly altered expression of CSC markers, radiosensitized CSCs to both X-ray and carbon ion beam, suggesting that XRCC4 may play a pivotal role in modulating cancer cell stemness. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第72回日本癌学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-10-05 | |||||
| 日付タイプ | Issued | |||||
