WEKO3
アイテム
XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強
https://repo.qst.go.jp/records/65241
https://repo.qst.go.jp/records/6524113f82cc8-f584-477c-a4eb-8fb3ca509f48
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-10-31 | |||||
タイトル | ||||||
タイトル | XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
崔, 星
× 崔, 星× 若井, 俊文× Guillaume, Vares× 王, 冰× 根井, 充× 上條, 岳彦× 白井, 敏之× 鎌田, 正× 崔 星× Guillaume Vares× 王 冰× 根井 充× 上條 岳彦× 白井 敏之× 鎌田 正 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Here we report the role of XRCC4 in cancer stem cell (CSC) properties and radiosensitivity of CSCs to X-ray and carbon ion beam. FACS analysis showed that the CSCs (CD133+, CD44+/ESA+) were significantly altered in XRCC4 KO cells compared to HCT116-WT cells. The number of colony and spheroid formed from CSCs were significantly higher compared to that from non-CSCs in XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. The proportion of CSCs was more extremely increased in XRCC4 KO cells compared to HCT116-WT cells after X-ray irradiation. Survival fraction analysis showed that the doses at D10 levels of CSCs sorted from XRCC4-KO and HCT116-WT cells were 1.4 and 4.2 Gy for X-ray and 1.0 and 1.9 Gy for carbon ion beam, respectively. A much more large number and large-sized gamma-H2AX foci were observed in CSCs sorted from XRCC4 KO cells compared to that from HCT116-WT cells, after 24 h carbon ion beam compared to X-ray irradiation. In conclusion, lack of XRCC4 significantly altered expression of CSC markers, radiosensitized CSCs to both X-ray and carbon ion beam, suggesting that XRCC4 may play a pivotal role in modulating cancer cell stemness. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 第72回日本癌学会 | |||||
発表年月日 | ||||||
日付 | 2013-10-05 | |||||
日付タイプ | Issued |