{"created":"2023-05-15T14:47:34.934838+00:00","id":65241,"links":{},"metadata":{"_buckets":{"deposit":"aa28428f-8f7b-4aae-8e5f-1db22918c43a"},"_deposit":{"created_by":1,"id":"65241","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65241"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065241","sets":["10:29"]},"author_link":["642780","642769","642777","642770","642771","642778","642768","642779","642774","642772","642773","642775","642781","642776","642767"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-10-05","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Here we report the role of XRCC4 in cancer stem cell (CSC) properties and radiosensitivity of CSCs to X-ray and carbon ion beam. FACS analysis showed that the CSCs (CD133+, CD44+/ESA+) were significantly altered in XRCC4 KO cells compared to HCT116-WT cells. The number of colony and spheroid formed from CSCs were significantly higher compared to that from non-CSCs in XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. The proportion of CSCs was more extremely increased in XRCC4 KO cells compared to HCT116-WT cells after X-ray irradiation. Survival fraction analysis showed that the doses at D10 levels of CSCs sorted from XRCC4-KO and HCT116-WT cells were 1.4 and 4.2 Gy for X-ray and 1.0 and 1.9 Gy for carbon ion beam, respectively. A much more large number and large-sized gamma-H2AX foci were observed in CSCs sorted from XRCC4 KO cells compared to that from HCT116-WT cells, after 24 h carbon ion beam compared to X-ray irradiation. In conclusion, lack of XRCC4 significantly altered expression of CSC markers, radiosensitized CSCs to both X-ray and carbon ion beam, suggesting that XRCC4 may play a pivotal role in modulating cancer cell stemness.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第72回日本癌学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"642767","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"若井, 俊文"}],"nameIdentifiers":[{"nameIdentifier":"642768","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ"}],"nameIdentifiers":[{"nameIdentifier":"642769","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王, 冰"}],"nameIdentifiers":[{"nameIdentifier":"642770","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井, 充"}],"nameIdentifiers":[{"nameIdentifier":"642771","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上條, 岳彦"}],"nameIdentifiers":[{"nameIdentifier":"642772","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"白井, 敏之"}],"nameIdentifiers":[{"nameIdentifier":"642773","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田, 正"}],"nameIdentifiers":[{"nameIdentifier":"642774","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642776","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642778","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上條 岳彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642779","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"白井 敏之","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642780","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"642781","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-31"},"publish_date":"2013-10-31","publish_status":"0","recid":"65241","relation_version_is_last":true,"title":["XRCC4遺伝子欠損によるヒト大腸癌幹細胞マーカー発現変化及びX線、炭素線に対する感受性増強"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:02:22.563678+00:00"}